Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS

J. H. Kang, R. Toita, Y. Jiang, T. Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Six peptides with various phosphorylation sensitivities for protein kinase A (PKA) were used for the simultaneous analysis of phosphorylated peptides using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. The mixture of six peptides was reacted with PKA and was analyzed by MALDI-TOF mass spectrometry. The intensity of all peaks except one phosphorylated peptide peak was very low (<20%). Moreover, we examined whether the addition of diammonium citrate to CHCA matrix at concentrations of 1-20 mg mL-1 can increase the peak intensity of peptides and phosphorylated peptides. The addition of diammonium citrate increased the peak intensity of peptides and phosphorylated peptides, but an increase in the intensity was unsatisfactory. Our study strongly suggests that MALDI-TOF mass spectrometry is not suitable for the simultaneous analysis of phosphorylated peptides.

元の言語英語
ページ(範囲)595-598
ページ数4
ジャーナルChromatographia
63
発行部数11-12
DOI
出版物ステータス出版済み - 6 1 2006

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Ionization
Desorption
Lasers
Peptides
Mass spectrometry
Mass Spectrometry
Cyclic AMP-Dependent Protein Kinases
Citric Acid
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Clinical Biochemistry

これを引用

Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS. / Kang, J. H.; Toita, R.; Jiang, Y.; Niidome, T.; Katayama, Yoshiki.

:: Chromatographia, 巻 63, 番号 11-12, 01.06.2006, p. 595-598.

研究成果: ジャーナルへの寄稿記事

Kang, J. H. ; Toita, R. ; Jiang, Y. ; Niidome, T. ; Katayama, Yoshiki. / Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS. :: Chromatographia. 2006 ; 巻 63, 番号 11-12. pp. 595-598.
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abstract = "Six peptides with various phosphorylation sensitivities for protein kinase A (PKA) were used for the simultaneous analysis of phosphorylated peptides using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. The mixture of six peptides was reacted with PKA and was analyzed by MALDI-TOF mass spectrometry. The intensity of all peaks except one phosphorylated peptide peak was very low (<20{\%}). Moreover, we examined whether the addition of diammonium citrate to CHCA matrix at concentrations of 1-20 mg mL-1 can increase the peak intensity of peptides and phosphorylated peptides. The addition of diammonium citrate increased the peak intensity of peptides and phosphorylated peptides, but an increase in the intensity was unsatisfactory. Our study strongly suggests that MALDI-TOF mass spectrometry is not suitable for the simultaneous analysis of phosphorylated peptides.",
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AU - Katayama, Yoshiki

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