Simultaneous Boron Ion-Channel/Growth Factor Receptor Activation for Enhanced Vascularization

Patricia Rico, Aleixandre Rodrigo-Navarro, Marcos de la Peña, Vladimira Moulisová, Mercedes Costell, Manuel Salmerón-Sánchez

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

Boron ion is essential in metabolism and its concentration is regulated by ion-channel NaBC1. NaBC1 mutations cause corneal dystrophies such as Harboyan syndrome. Here a 3D molecular model for NaBC1 is proposed and it is shown that simultaneous stimulation of NaBC1 and vascular endothelial growth factor receptors (VEGFR) promotes angiogenesis in vitro and in vivo with ultralow concentrations of VEGF. Human umbilical vein endothelial cells' (HUVEC) organization into tubular structures is shown to be indicative of vascularization potential. Enhanced cell sprouting is found only in the presence of VEGF and boron, the effect abrogated after blocking NaBC1. It is demonstrated that stimulated NaBC1 promotes angiogenesis via PI3k-independent pathways and that α5β1vβ3 integrin binding is not essential to enhanced HUVEC organization. A novel vascularization mechanism that involves crosstalk and colocalization between NaBC1 and VEGFR receptors is described. This has important translational consequences; just by administering boron, taking advantage of endogenous VEGF, in vivo vascularization is shown in a chorioallantoic membrane assay.

本文言語英語
論文番号1800220
ジャーナルAdvanced Biosystems
3
1
DOI
出版ステータス出版済み - 1月 2019
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生体材料
  • 生体医工学
  • 生化学、遺伝学、分子生物学(全般)

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