Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor

Xiaoguang Zhang, Sachiko Tsuji, Hayato Kitaoka, Hiroshi Kobayashi, Mitsuru Tamai, Ken Ichi Honjoh, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Abstract: Detection of foodborne pathogens at very low levels is still a challenge. A custom-built multichannel surface plasmon resonance (SPR) biosensor and simultaneous enrichment broth (SEB) were used to develop a simultaneous detection method for 3 important foodborne pathogens, Escherichia coli O157:H7 (O157:H7), Salmonella enteritidis, and Listeria monocytogenes, at a very low level. These 3 foodborne pathogens at a very low level (14, 6, and 28 CFU/25 g (mL) for O157:H7, S. enteritidis, and L. monocytogenes, respectively) were inoculated in SEB and incubated at 37 ˚C for 24 h. Sample prepared from the simultaneous enrichment culture was analyzed using the multichannel SPR biosensor and sensor chip immobilized with polyclonal antibodies specific to each of the target pathogens. O157:H7, S. enteritidis, and L. monocytogenes in chicken were detected simultaneously at an inoculum dose of 14, 6, and 28 CFU/25 g, respectively. Our method using a custom-built multichannel SPR biosensor and enrichment in SEB is expected as a rapid and simultaneous detection method for low levels of O157:H7, S. enteritidis, and L. monocytogenes in food. Practical Application: Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications.

元の言語英語
ページ(範囲)2357-2363
ページ数7
ジャーナルJournal of Food Science
82
発行部数10
DOI
出版物ステータス出版済み - 10 2017

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surface plasmon resonance
Salmonella enteritidis
Escherichia coli O157
Surface Plasmon Resonance
biosensors
Listeria monocytogenes
Biosensing Techniques
Salmonella Enteritidis
food pathogens
enrichment culture
Food Safety
pathogens
polyclonal antibodies
methodology
application methods
sensors (equipment)
food safety
Chickens
inoculum
chickens

All Science Journal Classification (ASJC) codes

  • Food Science

これを引用

Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor. / Zhang, Xiaoguang; Tsuji, Sachiko; Kitaoka, Hayato; Kobayashi, Hiroshi; Tamai, Mitsuru; Honjoh, Ken Ichi; Miyamoto, Takahisa.

:: Journal of Food Science, 巻 82, 番号 10, 10.2017, p. 2357-2363.

研究成果: ジャーナルへの寄稿記事

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abstract = "Abstract: Detection of foodborne pathogens at very low levels is still a challenge. A custom-built multichannel surface plasmon resonance (SPR) biosensor and simultaneous enrichment broth (SEB) were used to develop a simultaneous detection method for 3 important foodborne pathogens, Escherichia coli O157:H7 (O157:H7), Salmonella enteritidis, and Listeria monocytogenes, at a very low level. These 3 foodborne pathogens at a very low level (14, 6, and 28 CFU/25 g (mL) for O157:H7, S. enteritidis, and L. monocytogenes, respectively) were inoculated in SEB and incubated at 37 ˚C for 24 h. Sample prepared from the simultaneous enrichment culture was analyzed using the multichannel SPR biosensor and sensor chip immobilized with polyclonal antibodies specific to each of the target pathogens. O157:H7, S. enteritidis, and L. monocytogenes in chicken were detected simultaneously at an inoculum dose of 14, 6, and 28 CFU/25 g, respectively. Our method using a custom-built multichannel SPR biosensor and enrichment in SEB is expected as a rapid and simultaneous detection method for low levels of O157:H7, S. enteritidis, and L. monocytogenes in food. Practical Application: Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications.",
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AU - Zhang, Xiaoguang

AU - Tsuji, Sachiko

AU - Kitaoka, Hayato

AU - Kobayashi, Hiroshi

AU - Tamai, Mitsuru

AU - Honjoh, Ken Ichi

AU - Miyamoto, Takahisa

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