Simultaneous determination of aminopyrine hydroxylation and aminopyrine N-demethylation in liver microsomes by high-performance liquid chromatography

Kazuhide Inoue, Kannosuke Fujimori, Keinosuke Mizokami, Momoko Sunouchi, Akira Takanaka, Yoshihito Omori

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

Aminopyrine and its metabolites, including 3-hydroxymethyl-2-methyl-4-dimethylamino-1-phenyl-3-pyrazoline-5-one which is a hydroxylated metabolite of aminopyrine, were separated on a reversed-phase (C8) Radial-Pak column using a mobile phase of methanol-triethylamine-water (30:1:69) adjusted to pH 5.40 with acetic acid. Detection of the peak was performed by an ultraviolet detector at 254 nm. By the rapid and simple method, aminopyrine hydroxylation as well as aminopyrine N-demethylation in liver microsomes can be examined simultaneously.

本文言語英語
ページ(範囲)201-208
ページ数8
ジャーナルJournal of Chromatography B: Biomedical Sciences and Applications
274
C
DOI
出版ステータス出版済み - 1983
外部発表はい

All Science Journal Classification (ASJC) codes

  • 化学 (全般)

フィンガープリント

「Simultaneous determination of aminopyrine hydroxylation and aminopyrine N-demethylation in liver microsomes by high-performance liquid chromatography」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル