TY - JOUR
T1 - Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography-mass spectrometry
AU - Kage, Shigetoshi
AU - Kudo, Keiko
AU - Ikeda, Hideaki
AU - Ikeda, Noriaki
PY - 2004/6/5
Y1 - 2004/6/5
N2 - We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography-mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography-mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02 mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0 mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.
AB - We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography-mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography-mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02 mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0 mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.
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U2 - 10.1016/j.jchromb.2004.02.029
DO - 10.1016/j.jchromb.2004.02.029
M3 - Article
C2 - 15113546
AN - SCOPUS:2042446432
VL - 805
SP - 113
EP - 117
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1
ER -