Simultaneous determination of triazolam and its metabolites in human blood and urine by gas chromatography/mass spectrometry

Keiko Kudo, Noriaki Ikeda, Yukiko Hino

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

A sensitive and selective method has been developed for simultaneous determination of triazolam and its major metabolites, α-hydroxytriazolam and 4-hydroxytriazolam, in human whole blood and urine. The drugs were effectively extracted using a 3-step solvent extraction procedure followed by tert- butyl-dimethylsilyl derivatization, and subjected to gas chromatography-mass spectrometry in the negative ion chemical ionization mode. Estazolam was used as an internal standard. The calibration curve for each compound was linear in the range from 0.5 to 100 ng/g, and the lower limit of detection was 0.1 ng/g for whole blood and 0.2 ng/g for urine. Within-day precision of this method was evaluated in whole blood and urine samples at the concentration of 10 ng/g; the coefficient of within-day variation ranged from 2.7 to 10.8%.

本文言語英語
ページ(範囲)159-164
ページ数6
ジャーナルLegal Medicine
1
3
DOI
出版ステータス出版済み - 1999

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

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