Sinapyl alcohol-specific peroxidase isoenzyme catalyzes the formation of the dehydrogenative polymer from sinapyl alcohol

Wataru Aoyama, Shinya Sasaki, Shigeki Matsumura, Thoru Mitsunaga, Hirofumi Hirai, Yuji Tsutsumi, Tomoaki Nishida

研究成果: Contribution to journalArticle査読

41 被引用数 (Scopus)

抄録

Two peroxidases, CWPO-A and CWPO-C, were isolated from the cell walls of poplar (Populus alba L.) callus culture. The cationic CWPO-C showed a strong preference for sinapyl alcohol over coniferyl alcohol as substrate. Thus, the monolignol utilization of CWPO-C is unique compared with other peroxidases, including anionic CWPO-A and horseradish peroxidase (HRP). CWPO-C polymerized oligomeric sinapyl alcohol (S-oligo) and sinapyl alcohol, producing a polymer of greater molecular weight. In contrast, HRP, which is specific to coniferyl alcohol, produced sinapyl alcohol dimers, rather than catalyzing polymerization. Adding coniferyl alcohol as a radical mediator in the HRP-mediated reaction did not result in S-oligo polymerization. This report shows that CWPO-C is an isoenzyme specific to sinapyl alcohol that polymerizes oligomeric lignols. Its catalytic activity toward oligomeric lignols may be related to the lignification of angiosperm woody plant cell walls.

本文言語英語
ページ(範囲)497-504
ページ数8
ジャーナルJournal of Wood Science
48
6
DOI
出版ステータス出版済み - 2002

All Science Journal Classification (ASJC) codes

  • Biomaterials

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