Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants

Waraporn Putalun, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: 著書/レポートタイプへの貢献

抄録

A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

元の言語英語
ホスト出版物のタイトルApplications of Plant Metabolic Engineering
出版者Springer Netherlands
ページ283-295
ページ数13
9781402060311
ISBN(電子版)9781402060311
ISBN(印刷物)9781402060304
DOI
出版物ステータス出版済み - 1 1 2007
外部発表Yes

Fingerprint

Single-Chain Antibodies
Solanum aculeatissimum
secondary metabolites
genetically modified organisms
biosynthesis
antibodies
glycosides
recombinant antibodies
antigens
breeding methods
Glycosides
plant breeding
monoclonal antibodies
proteins
Escherichia coli
Solanum
Antigens
Immunoglobulin Fragments
breeding
Breeding

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

これを引用

Putalun, W., Tanaka, H., & Shoyama, Y. (2007). Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. : Applications of Plant Metabolic Engineering (巻 9781402060311, pp. 283-295). Springer Netherlands. https://doi.org/10.1007/978-1-4020-6031-1_12

Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. / Putalun, Waraporn; Tanaka, Hiroyuki; Shoyama, Yukihiro.

Applications of Plant Metabolic Engineering. 巻 9781402060311 Springer Netherlands, 2007. p. 283-295.

研究成果: 著書/レポートタイプへの貢献

Putalun, W, Tanaka, H & Shoyama, Y 2007, Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. : Applications of Plant Metabolic Engineering. 巻. 9781402060311, Springer Netherlands, pp. 283-295. https://doi.org/10.1007/978-1-4020-6031-1_12
Putalun W, Tanaka H, Shoyama Y. Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. : Applications of Plant Metabolic Engineering. 巻 9781402060311. Springer Netherlands. 2007. p. 283-295 https://doi.org/10.1007/978-1-4020-6031-1_12
Putalun, Waraporn ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. Applications of Plant Metabolic Engineering. 巻 9781402060311 Springer Netherlands, 2007. pp. 283-295
@inbook{a5ab539f38d64020adcf367ab7ecadda,
title = "Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants",
abstract = "A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.",
author = "Waraporn Putalun and Hiroyuki Tanaka and Yukihiro Shoyama",
year = "2007",
month = "1",
day = "1",
doi = "10.1007/978-1-4020-6031-1_12",
language = "English",
isbn = "9781402060304",
volume = "9781402060311",
pages = "283--295",
booktitle = "Applications of Plant Metabolic Engineering",
publisher = "Springer Netherlands",
address = "Netherlands",

}

TY - CHAP

T1 - Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants

AU - Putalun, Waraporn

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

PY - 2007/1/1

Y1 - 2007/1/1

N2 - A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

AB - A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

UR - http://www.scopus.com/inward/record.url?scp=84919629600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919629600&partnerID=8YFLogxK

U2 - 10.1007/978-1-4020-6031-1_12

DO - 10.1007/978-1-4020-6031-1_12

M3 - Chapter

SN - 9781402060304

VL - 9781402060311

SP - 283

EP - 295

BT - Applications of Plant Metabolic Engineering

PB - Springer Netherlands

ER -