Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants

Waraporn Putalun, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

本文言語英語
ホスト出版物のタイトルApplications of Plant Metabolic Engineering
出版社Springer Netherlands
ページ283-295
ページ数13
9781402060311
ISBN(電子版)9781402060311
ISBN(印刷版)9781402060304
DOI
出版ステータス出版済み - 1 1 2007

All Science Journal Classification (ASJC) codes

  • 農業および生物科学(全般)

フィンガープリント

「Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル