Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies

Judy E. Anderson, Ashley C. Wozniak, Wataru Mizunoya

研究成果: Chapter in Book/Report/Conference proceedingChapter

16 被引用数 (Scopus)

抄録

The technique of single muscle-fiber cultures has already proven valuable in extending knowledge of myogenesis, stem cell heterogeneity, the stem cell niche in skeletal muscle, and satellite cell activation. This report reviews the background of the model and applications, and details the procedures of muscle dissection, fiber digestion and isolation, cleaning the fiber preparation, plating fibers, and extensions of the technique for studying activation from stable quiescence of satellite cells, mRNA expression by in situ hybridization and regulation of satellite cell activation in zebrafish muscle by nitric oxide, hepatocyte growth factor.

本文言語英語
ホスト出版物のタイトルMyogenesis
ホスト出版物のサブタイトルMethods and Protocols
編集者Joseph DiMario
ページ85-102
ページ数18
DOI
出版ステータス出版済み - 2012

出版物シリーズ

名前Methods in Molecular Biology
798
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

フィンガープリント 「Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル