TY - CHAP
T1 - Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies
AU - Anderson, Judy E.
AU - Wozniak, Ashley C.
AU - Mizunoya, Wataru
PY - 2012
Y1 - 2012
N2 - The technique of single muscle-fiber cultures has already proven valuable in extending knowledge of myogenesis, stem cell heterogeneity, the stem cell niche in skeletal muscle, and satellite cell activation. This report reviews the background of the model and applications, and details the procedures of muscle dissection, fiber digestion and isolation, cleaning the fiber preparation, plating fibers, and extensions of the technique for studying activation from stable quiescence of satellite cells, mRNA expression by in situ hybridization and regulation of satellite cell activation in zebrafish muscle by nitric oxide, hepatocyte growth factor.
AB - The technique of single muscle-fiber cultures has already proven valuable in extending knowledge of myogenesis, stem cell heterogeneity, the stem cell niche in skeletal muscle, and satellite cell activation. This report reviews the background of the model and applications, and details the procedures of muscle dissection, fiber digestion and isolation, cleaning the fiber preparation, plating fibers, and extensions of the technique for studying activation from stable quiescence of satellite cells, mRNA expression by in situ hybridization and regulation of satellite cell activation in zebrafish muscle by nitric oxide, hepatocyte growth factor.
UR - http://www.scopus.com/inward/record.url?scp=84555191598&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84555191598&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-343-1_6
DO - 10.1007/978-1-61779-343-1_6
M3 - Chapter
C2 - 22130833
AN - SCOPUS:84555191598
SN - 9781617793424
T3 - Methods in Molecular Biology
SP - 85
EP - 102
BT - Myogenesis
A2 - DiMario, Joseph
ER -