Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan

Juliano De Oliveira Porciuncula, Takanori Furukawa, Kazuki Mori, Yosuke Shida, Hideki Hirakawa, Kosuke Tashiro, Satoru Kuhara, Satoshi Nakagawa, Yasushi Morikawa, Wataru Ogasawara

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of singlenucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.

元の言語英語
ページ(範囲)534-543
ページ数10
ジャーナルBioscience, Biotechnology and Biochemistry
77
発行部数3
DOI
出版物ステータス出版済み - 4 24 2013
外部発表Yes

Fingerprint

Trichoderma
Cellulase
Polymorphism
Mutagenesis
Single Nucleotide Polymorphism
Japan
Nucleotides
Ascomycota
Enzymes
Genes
Biomass
Fungi
Carbon
Genome
Phenotype
Industrial applications
Productivity

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan. / De Oliveira Porciuncula, Juliano; Furukawa, Takanori; Mori, Kazuki; Shida, Yosuke; Hirakawa, Hideki; Tashiro, Kosuke; Kuhara, Satoru; Nakagawa, Satoshi; Morikawa, Yasushi; Ogasawara, Wataru.

:: Bioscience, Biotechnology and Biochemistry, 巻 77, 番号 3, 24.04.2013, p. 534-543.

研究成果: ジャーナルへの寄稿記事

De Oliveira Porciuncula, J, Furukawa, T, Mori, K, Shida, Y, Hirakawa, H, Tashiro, K, Kuhara, S, Nakagawa, S, Morikawa, Y & Ogasawara, W 2013, 'Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan', Bioscience, Biotechnology and Biochemistry, 巻. 77, 番号 3, pp. 534-543. https://doi.org/10.1271/bbb.120794
De Oliveira Porciuncula, Juliano ; Furukawa, Takanori ; Mori, Kazuki ; Shida, Yosuke ; Hirakawa, Hideki ; Tashiro, Kosuke ; Kuhara, Satoru ; Nakagawa, Satoshi ; Morikawa, Yasushi ; Ogasawara, Wataru. / Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan. :: Bioscience, Biotechnology and Biochemistry. 2013 ; 巻 77, 番号 3. pp. 534-543.
@article{a70d790344214f579f06d84d32f14cf2,
title = "Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan",
abstract = "The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of singlenucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.",
author = "{De Oliveira Porciuncula}, Juliano and Takanori Furukawa and Kazuki Mori and Yosuke Shida and Hideki Hirakawa and Kosuke Tashiro and Satoru Kuhara and Satoshi Nakagawa and Yasushi Morikawa and Wataru Ogasawara",
year = "2013",
month = "4",
day = "24",
doi = "10.1271/bbb.120794",
language = "English",
volume = "77",
pages = "534--543",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "3",

}

TY - JOUR

T1 - Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan

AU - De Oliveira Porciuncula, Juliano

AU - Furukawa, Takanori

AU - Mori, Kazuki

AU - Shida, Yosuke

AU - Hirakawa, Hideki

AU - Tashiro, Kosuke

AU - Kuhara, Satoru

AU - Nakagawa, Satoshi

AU - Morikawa, Yasushi

AU - Ogasawara, Wataru

PY - 2013/4/24

Y1 - 2013/4/24

N2 - The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of singlenucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.

AB - The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of singlenucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.

UR - http://www.scopus.com/inward/record.url?scp=84876377014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876377014&partnerID=8YFLogxK

U2 - 10.1271/bbb.120794

DO - 10.1271/bbb.120794

M3 - Article

C2 - 23470758

AN - SCOPUS:84876377014

VL - 77

SP - 534

EP - 543

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 3

ER -