SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals

Sayaka Akieda-Asai, Nobuhiro Zaima, Koji Ikegami, Tomoaki Kahyo, Ikuko Yao, Takahiro Hatanaka, Shun ichiro Iemura, Rika Sugiyama, Takeaki Yokozeki, Yoshinobu Eishi, Morio Koike, Kyoji Ikeda, Takuya Chiba, Haruyoshi Yamaza, Isao Shimokawa, Si Young Song, Akira Matsuno, Akiko Mizutani, Motoji Sawabe, Moses V. ChaoMasashi Tanaka, Yasunori Kanaho, Tohru Natsume, Haruhiko Sugimura, Yukari Date, Michael W. Mcburney, Leonard Guarente, Mitsutoshi Setou

研究成果: ジャーナルへの寄稿記事

29 引用 (Scopus)

抄録

Background: SIRT1, a NAD-dependent deacetylase, has diverse roles in a variety of organs such as regulation of endocrine function and metabolism. However, it remains to be addressed how it regulates hormone release there. Methodology/Principal Findings: Here, we report that SIRT1 is abundantly expressed in pituitary thyrotropes and regulates thyroid hormone secretion. Manipulation of SIRT1 level revealed that SIRT1 positively regulated the exocytosis of TSH-containing granules. Using LC/MS-based interactomics, phosphatidylinositol-4-phosphate 5-kinase (PIP5K)y was identified as a SIRT1 binding partner and deacetylation substrate. SIRT1 deacetylated two specific lysine residues (K265/K268) in PIP5Ky and enhanced PIP5Ky enzyme activity. SIRT1-mediated TSH secretion was abolished by PIP5Ky knockdown. SIRT1 knockdown decreased the levels of deacetylated PIP5Ky, PI(4,5)P2, and reduced the secretion of TSH from pituitary cells. These results were also observed in SIRT1-knockout mice. Conclusions/Significance: Our findings indicated that the control of TSH release by the SIRT1-PIP5Kγ pathway is important for regulating the metabolism of the whole body.

元の言語英語
記事番号e11755
ジャーナルPloS one
5
発行部数7
DOI
出版物ステータス出版済み - 8 16 2010

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thyrotropin
Mammals
phosphatidylinositols
Thyrotropin
Metabolism
Lysine
lysine
phosphotransferases (kinases)
secretion
Thyrotrophs
mammals
phosphates
metabolism
exocytosis
hormone secretion
Exocytosis
Enzyme activity
thyroid hormones
Thyroid Hormones
Knockout Mice

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals. / Akieda-Asai, Sayaka; Zaima, Nobuhiro; Ikegami, Koji; Kahyo, Tomoaki; Yao, Ikuko; Hatanaka, Takahiro; Iemura, Shun ichiro; Sugiyama, Rika; Yokozeki, Takeaki; Eishi, Yoshinobu; Koike, Morio; Ikeda, Kyoji; Chiba, Takuya; Yamaza, Haruyoshi; Shimokawa, Isao; Song, Si Young; Matsuno, Akira; Mizutani, Akiko; Sawabe, Motoji; Chao, Moses V.; Tanaka, Masashi; Kanaho, Yasunori; Natsume, Tohru; Sugimura, Haruhiko; Date, Yukari; Mcburney, Michael W.; Guarente, Leonard; Setou, Mitsutoshi.

:: PloS one, 巻 5, 番号 7, e11755, 16.08.2010.

研究成果: ジャーナルへの寄稿記事

Akieda-Asai, S, Zaima, N, Ikegami, K, Kahyo, T, Yao, I, Hatanaka, T, Iemura, SI, Sugiyama, R, Yokozeki, T, Eishi, Y, Koike, M, Ikeda, K, Chiba, T, Yamaza, H, Shimokawa, I, Song, SY, Matsuno, A, Mizutani, A, Sawabe, M, Chao, MV, Tanaka, M, Kanaho, Y, Natsume, T, Sugimura, H, Date, Y, Mcburney, MW, Guarente, L & Setou, M 2010, 'SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals', PloS one, 巻. 5, 番号 7, e11755. https://doi.org/10.1371/journal.pone.0011755
Akieda-Asai, Sayaka ; Zaima, Nobuhiro ; Ikegami, Koji ; Kahyo, Tomoaki ; Yao, Ikuko ; Hatanaka, Takahiro ; Iemura, Shun ichiro ; Sugiyama, Rika ; Yokozeki, Takeaki ; Eishi, Yoshinobu ; Koike, Morio ; Ikeda, Kyoji ; Chiba, Takuya ; Yamaza, Haruyoshi ; Shimokawa, Isao ; Song, Si Young ; Matsuno, Akira ; Mizutani, Akiko ; Sawabe, Motoji ; Chao, Moses V. ; Tanaka, Masashi ; Kanaho, Yasunori ; Natsume, Tohru ; Sugimura, Haruhiko ; Date, Yukari ; Mcburney, Michael W. ; Guarente, Leonard ; Setou, Mitsutoshi. / SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals. :: PloS one. 2010 ; 巻 5, 番号 7.
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abstract = "Background: SIRT1, a NAD-dependent deacetylase, has diverse roles in a variety of organs such as regulation of endocrine function and metabolism. However, it remains to be addressed how it regulates hormone release there. Methodology/Principal Findings: Here, we report that SIRT1 is abundantly expressed in pituitary thyrotropes and regulates thyroid hormone secretion. Manipulation of SIRT1 level revealed that SIRT1 positively regulated the exocytosis of TSH-containing granules. Using LC/MS-based interactomics, phosphatidylinositol-4-phosphate 5-kinase (PIP5K)y was identified as a SIRT1 binding partner and deacetylation substrate. SIRT1 deacetylated two specific lysine residues (K265/K268) in PIP5Ky and enhanced PIP5Ky enzyme activity. SIRT1-mediated TSH secretion was abolished by PIP5Ky knockdown. SIRT1 knockdown decreased the levels of deacetylated PIP5Ky, PI(4,5)P2, and reduced the secretion of TSH from pituitary cells. These results were also observed in SIRT1-knockout mice. Conclusions/Significance: Our findings indicated that the control of TSH release by the SIRT1-PIP5Kγ pathway is important for regulating the metabolism of the whole body.",
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T1 - SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals

AU - Akieda-Asai, Sayaka

AU - Zaima, Nobuhiro

AU - Ikegami, Koji

AU - Kahyo, Tomoaki

AU - Yao, Ikuko

AU - Hatanaka, Takahiro

AU - Iemura, Shun ichiro

AU - Sugiyama, Rika

AU - Yokozeki, Takeaki

AU - Eishi, Yoshinobu

AU - Koike, Morio

AU - Ikeda, Kyoji

AU - Chiba, Takuya

AU - Yamaza, Haruyoshi

AU - Shimokawa, Isao

AU - Song, Si Young

AU - Matsuno, Akira

AU - Mizutani, Akiko

AU - Sawabe, Motoji

AU - Chao, Moses V.

AU - Tanaka, Masashi

AU - Kanaho, Yasunori

AU - Natsume, Tohru

AU - Sugimura, Haruhiko

AU - Date, Yukari

AU - Mcburney, Michael W.

AU - Guarente, Leonard

AU - Setou, Mitsutoshi

PY - 2010/8/16

Y1 - 2010/8/16

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