Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Sho Hei Uchinomiya; Hiroshi Nonaka; Sho Hei Fujishima; Shinya Tsukiji; Akio Ojida; Itaru Hamachi

doi:10.1039/b912025d

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Sho Hei Uchinomiya, Hiroshi Nonaka, Sho Hei Fujishima, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

58 被引用数 (Scopus)

抄録

A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

本文言語	英語
ページ（範囲）	5880-5882
ページ数	3
ジャーナル	Chemical Communications
号	39
DOI	https://doi.org/10.1039/b912025d
出版ステータス	出版済み - 1月 1 2009
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

触媒
電子材料、光学材料、および磁性材料
セラミックおよび複合材料
化学 (全般)
表面、皮膜および薄膜
金属および合金
材料化学

文献へのアクセス

10.1039/b912025d

他のファイルとリンク

引用スタイル

@article{2d4497076a8c4f488591340e8b7ef72d,

title = "Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe",

abstract = "A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.",

author = "Uchinomiya, {Sho Hei} and Hiroshi Nonaka and Fujishima, {Sho Hei} and Shinya Tsukiji and Akio Ojida and Itaru Hamachi",

year = "2009",

month = jan,

day = "1",

doi = "10.1039/b912025d",

language = "English",

pages = "5880--5882",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "39",

}

TY - JOUR

T1 - Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

AU - Uchinomiya, Sho Hei

AU - Nonaka, Hiroshi

AU - Fujishima, Sho Hei

AU - Tsukiji, Shinya

AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

AB - A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

UR - http://www.scopus.com/inward/record.url?scp=70349682638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349682638&partnerID=8YFLogxK

U2 - 10.1039/b912025d

DO - 10.1039/b912025d

M3 - Article

C2 - 19787128

AN - SCOPUS:70349682638

SP - 5880

EP - 5882

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 39

ER -

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

抄録

!!!All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル