Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Shohei Uchinomiya, Hiroshi Nonaka, Sho Hei Fujishima, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿 › 記事

44 引用 (Scopus)

抄録

A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

元の言語英語
ページ（範囲）5880-5882
ページ数3
ジャーナルChemical Communications
発行部数39
DOI
出版物ステータス出版済み - 10 12 2009

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Labeling
Proteins
Histidine

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

これを引用

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. / Uchinomiya, Shohei; Nonaka, Hiroshi; Fujishima, Sho Hei; Tsukiji, Shinya; Ojida, Akio; Hamachi, Itaru.

：: Chemical Communications, 番号 39, 12.10.2009, p. 5880-5882.

研究成果: ジャーナルへの寄稿 › 記事

Uchinomiya, S, Nonaka, H, Fujishima, SH, Tsukiji, S, Ojida, A & Hamachi, I 2009, 'Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe', Chemical Communications, 番号 39, pp. 5880-5882. https://doi.org/10.1039/b912025d
Uchinomiya S, Nonaka H, Fujishima SH, Tsukiji S, Ojida A, Hamachi I. Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. Chemical Communications. 2009 10 12;(39):5880-5882. https://doi.org/10.1039/b912025d
Uchinomiya, Shohei ; Nonaka, Hiroshi ; Fujishima, Sho Hei ; Tsukiji, Shinya ; Ojida, Akio ; Hamachi, Itaru. / Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. ：: Chemical Communications. 2009 ; 番号 39. pp. 5880-5882.
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