Smad9 is a new type of transcriptional regulator in bone morphogenetic protein signaling

S. Tsukamoto, T. Mizuta, M. Fujimoto, S. Ohte, K. Osawa, A. Miyamoto, K. Yoneyama, E. Murata, A. Machiya, E. Jimi, S. Kokabu, T. Katagiri

研究成果: Contribution to journalArticle査読

60 被引用数 (Scopus)

抄録

Smad1, Smad5 and Smad9 (also known as Smad8) are activated by phosphorylation by bone morphogenetic protein (BMP)-bound type I receptor kinases. We examined the role of Smad1, Smad5, and Smad9 by creating constitutively active forms (SmadDVD). Transcriptional activity of Smad9DVD was lower than that of Smad1DVD or Smad5DVD, even though all three SmadDVDs associated with Smad4 and bound to the target DNA. The linker region of Smad9 was sufficient to reduce transcriptional activity. Smad9 expression was increased by the activation of BMP signaling, similar to that of inhibitory Smads (I-Smads), and Smad9 reduced BMP activity. In contrast to I-Smads, however, Smad9 did not inhibit the type I receptor kinase and suppressed the constitutively active Smad1DVD. Smad9 formed complexes with Smad1 and bound to DNA but suppressed the transcription of the target gene. Taken together, our findings suggest that Smad9 is a new type of transcriptional regulator in BMP signaling.

本文言語英語
論文番号7596
ジャーナルScientific reports
4
DOI
出版ステータス出版済み - 12 23 2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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