Small GTPase Sar1 is crucial for proglutelin and α-globulin export from the endoplasmic reticulum in rice endosperm

Lihong Tian, Ling Ling Dai, Zhi Jie Yin, Masako Fukuda, Toshihiro Kumamaru, Xiang Bai Dong, Xiu Ping Xu, Le Qing Qu

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

Rice seed storage proteins glutelin and α-globulin are synthesized in the endoplasmic reticulum (ER) and deposited in protein storage vacuoles (PSVs). Sar1, a small GTPase, acts as a molecular switch to regulate the assembly of coat protein complex II, which exports secretory protein from the ER to the Golgi apparatus. To reveal the route by which glutelin and α-globulin exit the ER, four putative Sar1 genes (OsSar1a/b/c/d) were cloned from rice, and transgenic rice were generated with Sar1 overexpressed or suppressed by RNA interference (RNAi) specifically in the endosperm under the control of the rice glutelin promoter. Overexpression or suppression of any OsSar1 did not alter the phenotype. However, simultaneous knockdown of OsSar1a/b/c resulted in floury and shrunken seeds, with an increased level of glutelin precursor and decreased level of the mature α- and β-subunit. OsSar1abc RNAi endosperm generated numerous, spherical, novel protein bodies with highly electron-dense matrixes containing both glutelin and α-globulin. Notably, the novel protein bodies were surrounded by ribosomes, showing that they were derived from the ER. Some of the ER-derived dense protein bodies were attached to a blebbing structure containing prolamin. These results indicated that OsSar1a/b/c play a crucial role in storage proteins exiting from the ER, with functional redundancy in rice endosperm, and glutelin and α-globulin transported together from the ER to the Golgi apparatus by a pathway mediated by coat protein complex II.

元の言語英語
ページ(範囲)2831-2845
ページ数15
ジャーナルJournal of Experimental Botany
64
発行部数10
DOI
出版物ステータス出版済み - 7 1 2013

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Endosperm
Monomeric GTP-Binding Proteins
Globulins
guanosinetriphosphatase
glutelins
Glutens
globulins
Endoplasmic Reticulum
endoplasmic reticulum
endosperm
rice
protein bodies
Proteins
Capsid Proteins
Golgi Apparatus
storage proteins
RNA Interference
Golgi apparatus
coat proteins
RNA interference

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

Small GTPase Sar1 is crucial for proglutelin and α-globulin export from the endoplasmic reticulum in rice endosperm. / Tian, Lihong; Dai, Ling Ling; Yin, Zhi Jie; Fukuda, Masako; Kumamaru, Toshihiro; Dong, Xiang Bai; Xu, Xiu Ping; Qu, Le Qing.

:: Journal of Experimental Botany, 巻 64, 番号 10, 01.07.2013, p. 2831-2845.

研究成果: ジャーナルへの寄稿記事

Tian, Lihong ; Dai, Ling Ling ; Yin, Zhi Jie ; Fukuda, Masako ; Kumamaru, Toshihiro ; Dong, Xiang Bai ; Xu, Xiu Ping ; Qu, Le Qing. / Small GTPase Sar1 is crucial for proglutelin and α-globulin export from the endoplasmic reticulum in rice endosperm. :: Journal of Experimental Botany. 2013 ; 巻 64, 番号 10. pp. 2831-2845.
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abstract = "Rice seed storage proteins glutelin and α-globulin are synthesized in the endoplasmic reticulum (ER) and deposited in protein storage vacuoles (PSVs). Sar1, a small GTPase, acts as a molecular switch to regulate the assembly of coat protein complex II, which exports secretory protein from the ER to the Golgi apparatus. To reveal the route by which glutelin and α-globulin exit the ER, four putative Sar1 genes (OsSar1a/b/c/d) were cloned from rice, and transgenic rice were generated with Sar1 overexpressed or suppressed by RNA interference (RNAi) specifically in the endosperm under the control of the rice glutelin promoter. Overexpression or suppression of any OsSar1 did not alter the phenotype. However, simultaneous knockdown of OsSar1a/b/c resulted in floury and shrunken seeds, with an increased level of glutelin precursor and decreased level of the mature α- and β-subunit. OsSar1abc RNAi endosperm generated numerous, spherical, novel protein bodies with highly electron-dense matrixes containing both glutelin and α-globulin. Notably, the novel protein bodies were surrounded by ribosomes, showing that they were derived from the ER. Some of the ER-derived dense protein bodies were attached to a blebbing structure containing prolamin. These results indicated that OsSar1a/b/c play a crucial role in storage proteins exiting from the ER, with functional redundancy in rice endosperm, and glutelin and α-globulin transported together from the ER to the Golgi apparatus by a pathway mediated by coat protein complex II.",
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AU - Tian, Lihong

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AU - Fukuda, Masako

AU - Kumamaru, Toshihiro

AU - Dong, Xiang Bai

AU - Xu, Xiu Ping

AU - Qu, Le Qing

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