Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

Yusuke Ohnishi, Yasushi Totoki, Atsushi Toyoda, Toshiaki Watanabe, Yasuhiro Yamamoto, Katsushi Tokunaga, Yoshiyuki Sakaki, Hiroyuki Sasaki, Hirohiko Hohjoh

研究成果: Contribution to journalArticle査読

100 被引用数 (Scopus)

抄録

Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.

本文言語英語
論文番号gkq229
ページ(範囲)5141-5151
ページ数11
ジャーナルNucleic acids research
38
15
DOI
出版ステータス出版済み - 4 12 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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