Snapshot of a Michaelis complex in a sulfuryl transfer reaction

Crystal structure of a mouse sulfotransferase, mSULT1D1, complexed with donor substrate and accepter substrate

Takamasa Teramoto, Yoichi Sakakibara, Ming Cheh Liu, Masahito Suiko, Makoto Kimura, Yoshimitsu Kakuta

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

We report the crystal structure of mouse sulfotransferase, mSULT1D1, complexed with donor substrate 3′-phosphoadenosine 5′-phosphosulfate and accepter substrate p-nitrophenol. The structure is the first report of the native Michaelis complex of sulfotransferase. In the structure, three proposed catalytic residues (Lys48, Lys106, and His108) were in proper positions for engaging in the sulfuryl transfer reaction. The data strongly support that the sulfuryl transfer reaction proceeds through an SN2-like in-line displacement mechanism.

元の言語英語
ページ(範囲)83-87
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
383
発行部数1
DOI
出版物ステータス出版済み - 5 22 2009

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Sulfotransferases
Crystal structure
Phosphoadenosine Phosphosulfate
Substrates
4-nitrophenol

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

これを引用

Snapshot of a Michaelis complex in a sulfuryl transfer reaction : Crystal structure of a mouse sulfotransferase, mSULT1D1, complexed with donor substrate and accepter substrate. / Teramoto, Takamasa; Sakakibara, Yoichi; Liu, Ming Cheh; Suiko, Masahito; Kimura, Makoto; Kakuta, Yoshimitsu.

:: Biochemical and Biophysical Research Communications, 巻 383, 番号 1, 22.05.2009, p. 83-87.

研究成果: ジャーナルへの寄稿記事

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AU - Kimura, Makoto

AU - Kakuta, Yoshimitsu

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