S/O-nanodispersion electrospun fiber mesh effective for sustained release of healthy plasmid DNA with the structural and functional integrity

Tatsuya Okuda, Yoshiro Tahara, Noriho Kamiya, Masahiro Goto, Satoru Kidoaki

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

Localized and sustained delivery of the therapeutic genes using a solid carrier matrix is a potential approach to develop highly curative treatments. Electrospun nanofiber mesh of biodegradable polymer has been applied extensively as a carrier for localized and sustained delivery of drugs, proteins, and DNA, but it remains difficult to release sufficient amounts of DNA while maintaining structural and functional integrity. To realize the stable sustained release of the healthy plasmid DNA (pDNA) from electrospun fiber mesh, a novel method was examined for loading pDNA into the fibers based on solid-in-oil (S/O) nanodispersion of pDNA in organic solvent for electrospinning polymer solution: S/O nanodispersion electrospinning. A prepared pDNA-loaded fiber mesh made of biodegradable polymer showed sustained release of pDNA without burst release. From luciferase activity-based in vitro transcription-translation assay, pDNA released from meshes of the S/O nanodispersion retained about 60% luciferase activity of control pDNA, whereas pDNA released from the meshes of simple mixing showed only about 5% activity, indicating that S/O nanodispersion electrospinning is effective for loading pDNA into electrospun fiber meshes while maintaining their healthy functions. Effectiveness of S/O nanodispersion electrospinning was verified for fabricating a sustained release carrier matrix for high molecular weight bioactives, including therapeutic genes.

本文言語英語
ページ(範囲)1277-1290
ページ数14
ジャーナルJournal of Biomaterials Science, Polymer Edition
24
10
DOI
出版ステータス出版済み - 7月 1 2013

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • バイオエンジニアリング
  • 生体材料
  • 生体医工学

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