Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression

Hiroaki Mon, Zhiqing Li, Isao Kobayashi, Shuichiro Tomita, Jae Man Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

RNA interference (RNAi) is an evolutionarily conserved mechanism for sequence-specific gene silencing. Previously, the BmN4-SID1 cell expressing Caenorhabditis ele gans SID-1 was established, in which soaking RNAi could induce effective gene silencing. To establish its utility, 6 cell cycle progression related cDNAs, CDK1, MYC, MYB, RNRS, CDT1, and GEMININ, were isolated from the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), and their expressions were further silenced by soaking RNAi in the BmN4-SID1 cells. The cell cycle progression analysis using flow cytometer demonstrated that the small amount of double stranded RNA was enough to arrest cell cycle progression at the specific cell phases. These data suggest that RNAi in the BmN4-SID1 cells can be used as a powerful tool for loss-of-function analysis of B. mori genes.

本文言語英語
論文番号155
ジャーナルJournal of Insect Science
13
DOI
出版ステータス出版済み - 12 2013

All Science Journal Classification (ASJC) codes

  • 昆虫科学

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