Sodium-coupled glucose transporter as a functional glucose sensor of retinal microvascular circulation

Masanori Wakisaka, Takanari Kitazono, Masako Kato, Udai Nakamura, Maki Yoshioka, Yuji Uchizono, Mototaka Yoshinari

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

To clarify the function of the Na+-coupled glucose transporter in the regulation of cellular tone of cultured retinal pericytes, we investigated the effects of extracellular glucose concentration on cell size. The surface area and diameter of cultured bovine retinal pericytes under different glucose concentrations were measured by using a light microscope with a digital camera. We also examined the effects of extracellular Na+ and Ca2+, inhibitors of the Na+-coupled glucose transporter and Na+-Ca2+ exchanger, a Ca2+ channel blocker, and nonmetabolizable sugars on cell size. The surface area and diameter of the cells changed according to extracellular glucose concentrations. α-Methyl glucoside, which enters the cell through the Na+-coupled glucose transporter, induced cellular contraction. However, the cells did not contract in response to 2-deoxyglucose, which enters the cell through a facilitated glucose transporter. Glucose-induced cellular contraction was abolished in the absence of extracellular Na+ and Ca2+. Moreover, phlorizin, an inhibitor of the Na+-coupled glucose transporter, and 2′,4′-dichlorobenzamil-HCl, an inhibitor of the Na+-Ca2+ exchanger, also abolished glucose-induced cellular contraction, whereas nicardipine, a Ca2+ channel blocker, did not. Our results indicate that high extracellular glucose concentrations induce contraction of bovine retinal pericytes via Na+ entry through a Na+-coupled glucose transporter, suggesting that the Na+-coupled glucose transporter may act as a functional glucose sensor of retinal microvascular circulation.

元の言語英語
ページ(範囲)1183-1188
ページ数6
ジャーナルCirculation research
88
発行部数11
DOI
出版物ステータス出版済み - 6 8 2001

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Sodium-Glucose Transport Proteins
Facilitative Glucose Transport Proteins
Glucose
Pericytes
Cell Size
Phlorhizin
Nicardipine
Deoxyglucose
Glucosides
Light

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

これを引用

Sodium-coupled glucose transporter as a functional glucose sensor of retinal microvascular circulation. / Wakisaka, Masanori; Kitazono, Takanari; Kato, Masako; Nakamura, Udai; Yoshioka, Maki; Uchizono, Yuji; Yoshinari, Mototaka.

:: Circulation research, 巻 88, 番号 11, 08.06.2001, p. 1183-1188.

研究成果: ジャーナルへの寄稿記事

Wakisaka, Masanori ; Kitazono, Takanari ; Kato, Masako ; Nakamura, Udai ; Yoshioka, Maki ; Uchizono, Yuji ; Yoshinari, Mototaka. / Sodium-coupled glucose transporter as a functional glucose sensor of retinal microvascular circulation. :: Circulation research. 2001 ; 巻 88, 番号 11. pp. 1183-1188.
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