抄録
PCR single-stranded conformational polymorphism (SSCP) analysis is a simple and rapid electrophoretic technique for the sensitive detection of sequence variants of PCR products. Here we describe a cross-platform program package, quantitative interpretation of SSCP in capillary array (QUISCA), which allows semi-automated quantitative detection of sequence variants separated by multicolor fluorescence-based SSCP electrophoresis using various capillary array apparatus. The program, together with the QUISCAview as a graphical user interface, takes trace data in ASCII format and processes them with three modules: signal denoising/baseline subtraction, color-matrix construction/application, and calibration of peak positions between multiple capillary runs using internal standard peaks. QUISCA is compatible with data from various widely used capillary array sequencers and is suitable not only for finding or typing SNPs in individual DNAs but also for the accurate estimation of the allele frequencies of many SNPs using a pooled DNA strategy. QUISCA can also serve as a versatile core program for various fragment analyses.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 1342-1348 |
| ページ数 | 7 |
| ジャーナル | BioTechniques |
| 巻 | 33 |
| 発行部数 | 6 |
| 出版物ステータス | 出版済み - 12 1 2002 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
これを引用
Software for machine-independent quantitative interpretation of SSCP in capillary array electrophoresis (QUISCA). / Higasa, Koichiro; Kukita, Yoji; Baba, Shingo; Hayashi, Kenshi.
：: BioTechniques, 巻 33, 番号 6, 01.12.2002, p. 1342-1348.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Software for machine-independent quantitative interpretation of SSCP in capillary array electrophoresis (QUISCA)
AU - Higasa, Koichiro
AU - Kukita, Yoji
AU - Baba, Shingo
AU - Hayashi, Kenshi
PY - 2002/12/1
Y1 - 2002/12/1
N2 - PCR single-stranded conformational polymorphism (SSCP) analysis is a simple and rapid electrophoretic technique for the sensitive detection of sequence variants of PCR products. Here we describe a cross-platform program package, quantitative interpretation of SSCP in capillary array (QUISCA), which allows semi-automated quantitative detection of sequence variants separated by multicolor fluorescence-based SSCP electrophoresis using various capillary array apparatus. The program, together with the QUISCAview as a graphical user interface, takes trace data in ASCII format and processes them with three modules: signal denoising/baseline subtraction, color-matrix construction/application, and calibration of peak positions between multiple capillary runs using internal standard peaks. QUISCA is compatible with data from various widely used capillary array sequencers and is suitable not only for finding or typing SNPs in individual DNAs but also for the accurate estimation of the allele frequencies of many SNPs using a pooled DNA strategy. QUISCA can also serve as a versatile core program for various fragment analyses.
AB - PCR single-stranded conformational polymorphism (SSCP) analysis is a simple and rapid electrophoretic technique for the sensitive detection of sequence variants of PCR products. Here we describe a cross-platform program package, quantitative interpretation of SSCP in capillary array (QUISCA), which allows semi-automated quantitative detection of sequence variants separated by multicolor fluorescence-based SSCP electrophoresis using various capillary array apparatus. The program, together with the QUISCAview as a graphical user interface, takes trace data in ASCII format and processes them with three modules: signal denoising/baseline subtraction, color-matrix construction/application, and calibration of peak positions between multiple capillary runs using internal standard peaks. QUISCA is compatible with data from various widely used capillary array sequencers and is suitable not only for finding or typing SNPs in individual DNAs but also for the accurate estimation of the allele frequencies of many SNPs using a pooled DNA strategy. QUISCA can also serve as a versatile core program for various fragment analyses.
UR - http://www.scopus.com/inward/record.url?scp=0036917774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036917774&partnerID=8YFLogxK
M3 - Article
C2 - 12503322
AN - SCOPUS:0036917774
VL - 33
SP - 1342
EP - 1348
JO - BioTechniques
JF - BioTechniques
SN - 0736-6205
IS - 6
ER -