Soluble branched (1,4)-β-D-glucans from Acetobacter species enhance antitumor activities against MHC class I-negative and -positive malignant melanoma through augmented NK activity and cytotoxic T-cell response

Yoriyuki Kamiryo, Toshiki Yajima, Kimika Saito, Hitoshi Nishimura, Takashi Fushimi, Yoshifumi Ohshima, Yoshinori Tsukamoto, Seiji Naito, Yasunobu Yoshikai

    研究成果: Contribution to journalArticle査読

    18 被引用数 (Scopus)

    抄録

    We previously found that an extracellular polysaccharide, AC-1, produced by Acetobacter polysaccharogenes composed of (1,4)-β-D-glucan with branches of glucosyl residues showed a strong activity to induce production of interleukin (IL)-12 p40 and tumor necrosis factor-α by macrophage cell lines in vitro via Toll-like receptor-4 signaling. In the present study, we examined the effects of oral administration of AC-1 on protection against 2 types of murine B16 melanoma lines, major histocompatibility complex (MHC) class I-negative B16L and MHC class I gene-transfected B16Kb cells. Mice were inoculated subcutaneously with B16L or B16Kb cells on day 0 and administrated intragastrically with AC-1 or PBS once every 5 days from 1 day before tumor inoculation. The tumor growth was severely retarded in AC-1-treated mice after subcutaneous inoculation with B16L or B16Kb cells. The AC-1-treated mice showed augmented natural killer (NK) cell activity against B16L cells, and in vivo depletion of NK cells by antiasialoGM1 antibody (Ab) treatment abrogated the antitumor activity in AC-1-treated mice. On the other hand, AC-1-treated mice inoculated with B16Kb cells developed a significantly higher level of cytotoxic T-lymphocyte response against B16K b cells, and in vivo depletion of CD8+ T cells by anti-CD8 mAb treatment abrogated the antitumor activity. Thus, AC-1 augmented antitumor activity against different tumors via augmentation of different antitumor mechanisms. These results suggest a possible prophylactic application of AC-1 for human neoplasms irrespective of expression levels of their MHC class I molecules.

    本文言語英語
    ページ(範囲)769-776
    ページ数8
    ジャーナルInternational Journal of Cancer
    115
    5
    DOI
    出版ステータス出版済み - 7 10 2005

    All Science Journal Classification (ASJC) codes

    • 腫瘍学
    • 癌研究

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