TY - JOUR
T1 - Soybean antiviral immunity conferred by dsRNase targets the viral replication complex
AU - Ishibashi, Kazuhiro
AU - Saruta, Masayasu
AU - Shimizu, Takehiko
AU - Shu, Miao
AU - Anai, Toyoaki
AU - Komatsu, Kunihiko
AU - Yamada, Naohiro
AU - Katayose, Yuichi
AU - Ishikawa, Masayuki
AU - Ishimoto, Masao
AU - Kaga, Akito
N1 - Funding Information:
We thank S. Namba, Y. Yamaji, K. Maejima, and the Genetic Resources Center, NARO, for providing leaves infected with PPV and other potyviruses. This work was supported in part by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, SFC-1008) and from the Genebank Project, NARO.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Eukaryotic positive-strand RNA viruses replicate their genomes in membranous compartments formed in a host cell, which sequesters the dsRNA replication intermediate from antiviral immune surveillance. Here, we find that soybean has developed a way to overcome this sequestration. We report the positional cloning of the broad-spectrum soybean mosaic virus resistance gene Rsv4, which encodes an RNase H family protein with dsRNA-degrading activity. An active-site mutant of Rsv4 is incapable of inhibiting virus multiplication and is associated with an active viral RNA polymerase complex in infected cells. These results suggest that Rsv4 enters the viral replication compartment and degrades viral dsRNA. Inspired by this model, we design three plant-gene-derived dsRNases that can inhibit the multiplication of the respective target viruses. These findings suggest a method for developing crops resistant to any target positive-strand RNA virus by fusion of endogenous host genes.
AB - Eukaryotic positive-strand RNA viruses replicate their genomes in membranous compartments formed in a host cell, which sequesters the dsRNA replication intermediate from antiviral immune surveillance. Here, we find that soybean has developed a way to overcome this sequestration. We report the positional cloning of the broad-spectrum soybean mosaic virus resistance gene Rsv4, which encodes an RNase H family protein with dsRNA-degrading activity. An active-site mutant of Rsv4 is incapable of inhibiting virus multiplication and is associated with an active viral RNA polymerase complex in infected cells. These results suggest that Rsv4 enters the viral replication compartment and degrades viral dsRNA. Inspired by this model, we design three plant-gene-derived dsRNases that can inhibit the multiplication of the respective target viruses. These findings suggest a method for developing crops resistant to any target positive-strand RNA virus by fusion of endogenous host genes.
UR - http://www.scopus.com/inward/record.url?scp=85072707115&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072707115&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-12052-5
DO - 10.1038/s41467-019-12052-5
M3 - Article
C2 - 31562302
AN - SCOPUS:85072707115
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 4033
ER -