Soybean antiviral immunity conferred by dsRNase targets the viral replication complex

Kazuhiro Ishibashi, Masayasu Saruta, Takehiko Shimizu, Miao Shu, Toyoaki Anai, Kunihiko Komatsu, Naohiro Yamada, Yuichi Katayose, Masayuki Ishikawa, Masao Ishimoto, Akito Kaga

研究成果: ジャーナルへの寄稿学術誌査読

27 被引用数 (Scopus)

抄録

Eukaryotic positive-strand RNA viruses replicate their genomes in membranous compartments formed in a host cell, which sequesters the dsRNA replication intermediate from antiviral immune surveillance. Here, we find that soybean has developed a way to overcome this sequestration. We report the positional cloning of the broad-spectrum soybean mosaic virus resistance gene Rsv4, which encodes an RNase H family protein with dsRNA-degrading activity. An active-site mutant of Rsv4 is incapable of inhibiting virus multiplication and is associated with an active viral RNA polymerase complex in infected cells. These results suggest that Rsv4 enters the viral replication compartment and degrades viral dsRNA. Inspired by this model, we design three plant-gene-derived dsRNases that can inhibit the multiplication of the respective target viruses. These findings suggest a method for developing crops resistant to any target positive-strand RNA virus by fusion of endogenous host genes.

本文言語英語
論文番号4033
ジャーナルNature communications
10
1
DOI
出版ステータス出版済み - 12月 1 2019
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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