Soybean milk-based extender for cryopreservation of buck spermatozoa

Waleed Soltan, El Shenawy El-Siefy, Ibrahim Abd El-Razek, Mohamed El-Sharawy, Kaiyu Kubota, Nobuhiko Yamauchi, Ibrahim El-Shamaa

研究成果: ジャーナルへの寄稿記事

抄録

The aim of this study was to investigate incorporating whole soybean milk instead of the traditional egg yolk in goat semen extender. The semen ejaculates were collected from three fertile bucks, aged 2-3 years using artificial vagina. Collected semen was divided into 6 parts; the first was diluted with Tris-egg yolk extender (TEY) saved as control, while the others were diluted with Tris-soybean milk extender (TSM) at levels of 5, 10, 15, 20 and 25%. Pooled ejaculates were further processed for freezing using 0.25 ml French straws. Diluted semen at a rate of 1:4 was placed into a refrigerator at 5°C for 4 h as equilibration period. At the end of equilibration period, extended semen was packaged in straws and stored at -196°C. Then after, frozen semen was thawed by dipping the straws into a water bath at 37°C for 30 seconds. Percentages of sperm motility, live spermatozoa, sperm abnormalities and recovery rate were determined. The results revealed that there were significant differences (P<0.05) in buck sperm characteristics (percentages of sperm motility, live spermatozoa, sperm abnormalities and plasma membrane and acrosome integrity) among post-dilution, post equilibration and post thawing processes. Addition of 15% of soybean milk led to a significant (P<0.05) improvement of sperm motility, live spermatozoa, sperm abnormalities and plasma membrane and acrosome integrity of buck spermatozoa during different stages of cryopreservation compared to control, while the lowest values were recorded at a level of 25% soybean milk extender. On the basis of our results, we concluded that soybean milk-based extender at the rate of 15% of soybean milk has the potential to maintain buck sperm quality after freezing-thawing process compared with cryopreservation in a traditional protection extender (egg yolk).

元の言語英語
ページ(範囲)361-366
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
62
発行部数2
出版物ステータス出版済み - 9 1 2017

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soymilk
bucks
Cryopreservation
Soybeans
cryopreservation
Spermatozoa
Milk
spermatozoa
Semen
semen
Egg Yolk
Sperm Motility
sperm motility
egg yolk
straw
Acrosome
acrosome
Freezing
plasma membrane
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Soltan, W., El-Siefy, E. S., El-Razek, I. A., El-Sharawy, M., Kubota, K., Yamauchi, N., & El-Shamaa, I. (2017). Soybean milk-based extender for cryopreservation of buck spermatozoa. Journal of the Faculty of Agriculture, Kyushu University, 62(2), 361-366.

Soybean milk-based extender for cryopreservation of buck spermatozoa. / Soltan, Waleed; El-Siefy, El Shenawy; El-Razek, Ibrahim Abd; El-Sharawy, Mohamed; Kubota, Kaiyu; Yamauchi, Nobuhiko; El-Shamaa, Ibrahim.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 62, 番号 2, 01.09.2017, p. 361-366.

研究成果: ジャーナルへの寄稿記事

Soltan, W, El-Siefy, ES, El-Razek, IA, El-Sharawy, M, Kubota, K, Yamauchi, N & El-Shamaa, I 2017, 'Soybean milk-based extender for cryopreservation of buck spermatozoa', Journal of the Faculty of Agriculture, Kyushu University, 巻. 62, 番号 2, pp. 361-366.
Soltan W, El-Siefy ES, El-Razek IA, El-Sharawy M, Kubota K, Yamauchi N その他. Soybean milk-based extender for cryopreservation of buck spermatozoa. Journal of the Faculty of Agriculture, Kyushu University. 2017 9 1;62(2):361-366.
Soltan, Waleed ; El-Siefy, El Shenawy ; El-Razek, Ibrahim Abd ; El-Sharawy, Mohamed ; Kubota, Kaiyu ; Yamauchi, Nobuhiko ; El-Shamaa, Ibrahim. / Soybean milk-based extender for cryopreservation of buck spermatozoa. :: Journal of the Faculty of Agriculture, Kyushu University. 2017 ; 巻 62, 番号 2. pp. 361-366.
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title = "Soybean milk-based extender for cryopreservation of buck spermatozoa",
abstract = "The aim of this study was to investigate incorporating whole soybean milk instead of the traditional egg yolk in goat semen extender. The semen ejaculates were collected from three fertile bucks, aged 2-3 years using artificial vagina. Collected semen was divided into 6 parts; the first was diluted with Tris-egg yolk extender (TEY) saved as control, while the others were diluted with Tris-soybean milk extender (TSM) at levels of 5, 10, 15, 20 and 25{\%}. Pooled ejaculates were further processed for freezing using 0.25 ml French straws. Diluted semen at a rate of 1:4 was placed into a refrigerator at 5°C for 4 h as equilibration period. At the end of equilibration period, extended semen was packaged in straws and stored at -196°C. Then after, frozen semen was thawed by dipping the straws into a water bath at 37°C for 30 seconds. Percentages of sperm motility, live spermatozoa, sperm abnormalities and recovery rate were determined. The results revealed that there were significant differences (P<0.05) in buck sperm characteristics (percentages of sperm motility, live spermatozoa, sperm abnormalities and plasma membrane and acrosome integrity) among post-dilution, post equilibration and post thawing processes. Addition of 15{\%} of soybean milk led to a significant (P<0.05) improvement of sperm motility, live spermatozoa, sperm abnormalities and plasma membrane and acrosome integrity of buck spermatozoa during different stages of cryopreservation compared to control, while the lowest values were recorded at a level of 25{\%} soybean milk extender. On the basis of our results, we concluded that soybean milk-based extender at the rate of 15{\%} of soybean milk has the potential to maintain buck sperm quality after freezing-thawing process compared with cryopreservation in a traditional protection extender (egg yolk).",
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