Spatial geometries of self-assembled chitohexaose monolayers regulate myoblast fusion

Pornthida Poosala, Hirofumi Ichinose, Takuya Kitaoka

研究成果: ジャーナルへの寄稿記事

抄録

Myoblast fusion into functionally-distinct myotubes to form in vitro skeletal muscle constructs under differentiation serum-free conditions still remains a challenge. Herein, we report that our microtopographical carbohydrate substrates composed of bioactive hexa-N-acetyl-D-glucosamine (GlcNAc6) modulated the efficiency of myoblast fusion without requiring horse serum or any differentiation medium during cell culture. Promotion of the differentiation of dissociated mononucleated skeletal myoblasts (C2C12; a mouse myoblast cell line) into robust myotubes was found only on GlcNAc6 micropatterns, whereas the myoblasts on control, non-patterned GlcNAc6 substrates or GlcNAc6-free patterns exhibited an undifferentiated form. We also examined the possible role of GlcNAc6 micropatterns with various widths in the behavior of C2C12 cells in early and late stages of myogenesis through mRNA expression of myosin heavy chain (MyHC) isoforms. The spontaneous contraction of myotubes was investigated via the regulation of glucose transporter type 4 (GLUT4), which is involved in stimulating glucose uptake during cellular contraction. Narrow patterns demonstrated enhanced glucose uptake rate and generated a fast-twitch muscle fiber type, whereas the slow-twitch muscle fiber type was dominant on wider patterns. Our findings indicated that GlcNAc6-mediated integrin interactions are responsible for guiding myoblast fusion forward along with myotube formation.

元の言語英語
記事番号686
ジャーナルInternational Journal of Molecular Sciences
17
発行部数5
DOI
出版物ステータス出版済み - 5 6 2016

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Myoblasts
Self assembled monolayers
glucose
muscle fibers
Skeletal Muscle Fibers
Glucose
Muscle
Fusion reactions
fusion
serums
contraction
Geometry
geometry
Glucose Transporter Type 4
Glucosamine
myosins
transporter
skeletal muscle
horses
Acetylglucosamine

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

これを引用

Spatial geometries of self-assembled chitohexaose monolayers regulate myoblast fusion. / Poosala, Pornthida; Ichinose, Hirofumi; Kitaoka, Takuya.

:: International Journal of Molecular Sciences, 巻 17, 番号 5, 686, 06.05.2016.

研究成果: ジャーナルへの寄稿記事

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