Spatiotemporal, allelic, and enforced expression of Ximpact, the Xenopus homolog of mouse imprinted gene Impact

Yoichi Yamada, Yuriko Hagiwara, Koichiro Shiokawa, Yoshiyuki Sakaki, Takashi Ito

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Mouse Impact is an imprinted gene encoding an evolutionarily conserved protein of unknown function. We isolated cDNA for the Xenopus homolog of Impact (Ximpact), since the clawed frog not only provides an excellent model for the study of gene function in early development but also allows the generation of interspecific F1 hybrids required for the examination of allelic expression status. The predicted product of Ximpact shows an extreme sequence similarity to those of mouse Impact and its homologs in nematoda, fission yeast, and budding yeast. The transcript of Ximpact is present in oocytes as well as in early embryos, and its spatial distribution is ubiquitous in both embryonic and adult stages. An RT-PCR-RFLP assay using the reciprocal interspecific F1 hybrids and a DNA polymorphism between X. laevis and X. borealis showed that Ximpact is expressed biallelically when analyzed as a whole embryo. Overexpression of Ximpact by RNA microinjection resulted in a higher than normal rate of gastrulation defects, suggesting the need for tight control of its dosage in early development.

元の言語英語
ページ(範囲)162-169
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
256
発行部数1
DOI
出版物ステータス出版済み - 3 5 1999
外部発表Yes

Fingerprint

Xenopus
Yeast
Genes
Gene encoding
Polymorphism
Spatial distribution
Assays
Complementary DNA
RNA
Embryonic Structures
Defects
DNA
Nematoda
Gastrulation
Saccharomycetales
Schizosaccharomyces
Microinjections
Proteins
Restriction Fragment Length Polymorphisms
Anura

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Spatiotemporal, allelic, and enforced expression of Ximpact, the Xenopus homolog of mouse imprinted gene Impact. / Yamada, Yoichi; Hagiwara, Yuriko; Shiokawa, Koichiro; Sakaki, Yoshiyuki; Ito, Takashi.

:: Biochemical and Biophysical Research Communications, 巻 256, 番号 1, 05.03.1999, p. 162-169.

研究成果: ジャーナルへの寄稿記事

Yamada, Yoichi ; Hagiwara, Yuriko ; Shiokawa, Koichiro ; Sakaki, Yoshiyuki ; Ito, Takashi. / Spatiotemporal, allelic, and enforced expression of Ximpact, the Xenopus homolog of mouse imprinted gene Impact. :: Biochemical and Biophysical Research Communications. 1999 ; 巻 256, 番号 1. pp. 162-169.
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abstract = "Mouse Impact is an imprinted gene encoding an evolutionarily conserved protein of unknown function. We isolated cDNA for the Xenopus homolog of Impact (Ximpact), since the clawed frog not only provides an excellent model for the study of gene function in early development but also allows the generation of interspecific F1 hybrids required for the examination of allelic expression status. The predicted product of Ximpact shows an extreme sequence similarity to those of mouse Impact and its homologs in nematoda, fission yeast, and budding yeast. The transcript of Ximpact is present in oocytes as well as in early embryos, and its spatial distribution is ubiquitous in both embryonic and adult stages. An RT-PCR-RFLP assay using the reciprocal interspecific F1 hybrids and a DNA polymorphism between X. laevis and X. borealis showed that Ximpact is expressed biallelically when analyzed as a whole embryo. Overexpression of Ximpact by RNA microinjection resulted in a higher than normal rate of gastrulation defects, suggesting the need for tight control of its dosage in early development.",
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AU - Sakaki, Yoshiyuki

AU - Ito, Takashi

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