Spatiotemporal distribution of PKCα, Cdc42, and Rac1 before directed cell migration

Saori Sasaki, Ryu Takahashi, Yangfeng Luo, Kengo Chujo, Toshihiro Sera, Susumu Kudo

研究成果: Contribution to journalArticle査読

抄録

Cdc42 is a key factor in directed cell migration and accumulates at the leading edge of migrating cells. However, what kind of proteins control Cdc42 and when is unclear. After mechanical wounding, protein kinase C α (PKCα), a conventional PKC isozyme, begins to accumulate at the edges of cells adjacent to the wounded cells (WCs). In this study, we hypothesized that PKCα may be implicated in directed cell migration at an early stage before Cdc42 controls the migration. We focused on the spatiotemporal distribution of PKCα, Cdc42, and Rac1 before cell migration. After wounding, at the edges of cells adjacent to the WCs, PKCα accumulation, Cdc42 accumulation, Rac1 accumulation, and filopodia formation occurred in that order. The PKCα inhibitor suppressed Cdc42 accumulation at the cell edges. These results suggest that inhibition of PKCα activity inhibits cell migration. In addition, it is not Cdc42 but PKCα that may decide the direction of cell migration.

本文言語英語
ページ(範囲)26-31
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
584
DOI
出版ステータス出版済み - 12 20 2021

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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