TY - JOUR
T1 - Species-specific neuronal localization of kynurenine aminotransferase-2 in the mouse cerebellum
AU - Balog, Emma
AU - Jenei, Gyula
AU - Gellért, Levente
AU - Ono, Etsuro
AU - Vécsei, László
AU - Toldi, József
AU - Kis, Zsolt
N1 - Funding Information:
This work was supported by grants GINOP-2.3.2-15-2016-00034 , JSPS-HAS (NKM-25/2019) , TUDFO/47138-1/2019-ITM , and the MTA-SZTE Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged . The authors are grateful to Péter Sántha for expert histological technical assistance, University of Szeged, Department of Physiology. This work was supported by the University of Szeged Open Access Fund , grant number: 5041 .
Publisher Copyright:
© 2020 The Authors
PY - 2021/1
Y1 - 2021/1
N2 - The immunohistochemical pattern of kynurenine aminotransferase-2 (KAT-2) - the key role enzyme in the production of neuroactive and neuroprotective kynurenic acid (KYNA) - was studied in the cerebellum of mice. It is known from literature that KAT-2 is localized mainly in astrocytes in different parts of the cerebrum. Kynurenine aminotransferase (KAT) activity in the cerebellum is relatively low and alternative production routes for KYNA have been described there. Therefore we examined the immunohistochemical pattern of KAT-2 in this part of the brain. Surprisingly, the cellular localization of KAT-2 in mice was proven to be unique; it localized characteristically in Purkinje cells and in some other types of neurons (not identified) but was not found in astrocytes nor microglia. The exclusive neuronal, but not glial localization of KAT-2 in the cerebellum is novel and may be related to its low activity and to the alternative pathways for KYNA production that have been described.
AB - The immunohistochemical pattern of kynurenine aminotransferase-2 (KAT-2) - the key role enzyme in the production of neuroactive and neuroprotective kynurenic acid (KYNA) - was studied in the cerebellum of mice. It is known from literature that KAT-2 is localized mainly in astrocytes in different parts of the cerebrum. Kynurenine aminotransferase (KAT) activity in the cerebellum is relatively low and alternative production routes for KYNA have been described there. Therefore we examined the immunohistochemical pattern of KAT-2 in this part of the brain. Surprisingly, the cellular localization of KAT-2 in mice was proven to be unique; it localized characteristically in Purkinje cells and in some other types of neurons (not identified) but was not found in astrocytes nor microglia. The exclusive neuronal, but not glial localization of KAT-2 in the cerebellum is novel and may be related to its low activity and to the alternative pathways for KYNA production that have been described.
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U2 - 10.1016/j.neuint.2020.104920
DO - 10.1016/j.neuint.2020.104920
M3 - Article
C2 - 33238153
AN - SCOPUS:85096964450
VL - 142
JO - Neurochemistry International
JF - Neurochemistry International
SN - 0197-0186
M1 - 104920
ER -