Specific hydrolysis of intact erythrocyte cell‐surface glycosphingolipids by endoglycoceramidase: Lack of modulation of erythrocyte glucose transporter by endogenous glycosphingolipids

Makoto Ito, Yuko IKEGAMI, Tadashi TAI, Tatuya YAMAGATA

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

This study represents the specific hydrolysis of cell‐surface glycosphingolipids (GSLs) of intact cells by endoglycoceramidase (EGCase; EC.3.2.1.123) which cleaves the linkage between oligosaccharides and ceramides of various GSLs. After a 2‐h incubation of horse intact erythrocytes with 20 mU EGCase II in the presence of activator at 37°C, 68% of the N‐glycolylneuraminic‐acid‐containing ganglioside GM3(NeuGc) and 70% of 4‐O‐acetyl GM3(NeuGc) were found to be hydrolyzed without hemolysis, accompanied by a corresponding increase in ceramide but not sphingosine or N,N‐dimethylsphingosine. No hydrolysis was observed for sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, cholesterol or membrane proteins. The decrease in immunoreactivity with GMR8 antibody, specific to NeuGcα2,3Gal‐ of GM3(NeuGc), corresponded to that of cell‐surface GM3(NeuGc) by the enzyme, and almost no immunoreactivity was found when 70% of the GM3(NeuGc) was hydrolyzed. Besides the cell‐surface GM3(NeuGc) of horse erythrocytes, Gg3Cer of guinea pig, GM3(NeuAc) and LcCer of human, and bovine and rabbit erythrocyte IV3Galα‐nLc4Cer were found to be efficiently hydrolyzed by EGCase II even when present in intact cells, while human erythrocyte Gb4Cer is quite resistant to hydrolysis by the enzyme on the cell surface as well as in detergent miscelles. Glucose incorporation via the glucose transporter in erythrocytes was not affected at all by the specific and exhaustive hydrolysis of cell‐surface GSLs by EGCase II. This result strongly suggested that glucose transporter function was not directly modulated by endogenous GSLs. In summary, this paper demonstrates that, together with the assistance of activator protein, EGCase II will become a powerful tool for selectively removing sugar chains from cell‐surface GSLs without damaging other cell membrane components, and will be useful for describing the biological functions of endogenous GSLs.

元の言語英語
ページ(範囲)637-643
ページ数7
ジャーナルEuropean Journal of Biochemistry
218
発行部数2
DOI
出版物ステータス出版済み - 1 1 1993

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endoglycoceramidase
Glucose Transporter Type 1
Glycosphingolipids
Hydrolysis
Erythrocytes
Modulation
Facilitative Glucose Transport Proteins
Ceramides
Horses
G(M3) Ganglioside
Sphingosine
Sphingomyelins
Enzymes
Cellular Structures
Cell membranes
Hemolysis
Phosphatidylcholines
Oligosaccharides
Sugars
Detergents

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

Specific hydrolysis of intact erythrocyte cell‐surface glycosphingolipids by endoglycoceramidase : Lack of modulation of erythrocyte glucose transporter by endogenous glycosphingolipids. / Ito, Makoto; IKEGAMI, Yuko; TAI, Tadashi; YAMAGATA, Tatuya.

:: European Journal of Biochemistry, 巻 218, 番号 2, 01.01.1993, p. 637-643.

研究成果: ジャーナルへの寄稿記事

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title = "Specific hydrolysis of intact erythrocyte cell‐surface glycosphingolipids by endoglycoceramidase: Lack of modulation of erythrocyte glucose transporter by endogenous glycosphingolipids",
abstract = "This study represents the specific hydrolysis of cell‐surface glycosphingolipids (GSLs) of intact cells by endoglycoceramidase (EGCase; EC.3.2.1.123) which cleaves the linkage between oligosaccharides and ceramides of various GSLs. After a 2‐h incubation of horse intact erythrocytes with 20 mU EGCase II in the presence of activator at 37°C, 68{\%} of the N‐glycolylneuraminic‐acid‐containing ganglioside GM3(NeuGc) and 70{\%} of 4‐O‐acetyl GM3(NeuGc) were found to be hydrolyzed without hemolysis, accompanied by a corresponding increase in ceramide but not sphingosine or N,N‐dimethylsphingosine. No hydrolysis was observed for sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, cholesterol or membrane proteins. The decrease in immunoreactivity with GMR8 antibody, specific to NeuGcα2,3Gal‐ of GM3(NeuGc), corresponded to that of cell‐surface GM3(NeuGc) by the enzyme, and almost no immunoreactivity was found when 70{\%} of the GM3(NeuGc) was hydrolyzed. Besides the cell‐surface GM3(NeuGc) of horse erythrocytes, Gg3Cer of guinea pig, GM3(NeuAc) and LcCer of human, and bovine and rabbit erythrocyte IV3Galα‐nLc4Cer were found to be efficiently hydrolyzed by EGCase II even when present in intact cells, while human erythrocyte Gb4Cer is quite resistant to hydrolysis by the enzyme on the cell surface as well as in detergent miscelles. Glucose incorporation via the glucose transporter in erythrocytes was not affected at all by the specific and exhaustive hydrolysis of cell‐surface GSLs by EGCase II. This result strongly suggested that glucose transporter function was not directly modulated by endogenous GSLs. In summary, this paper demonstrates that, together with the assistance of activator protein, EGCase II will become a powerful tool for selectively removing sugar chains from cell‐surface GSLs without damaging other cell membrane components, and will be useful for describing the biological functions of endogenous GSLs.",
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T2 - Lack of modulation of erythrocyte glucose transporter by endogenous glycosphingolipids

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AU - IKEGAMI, Yuko

AU - TAI, Tadashi

AU - YAMAGATA, Tatuya

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N2 - This study represents the specific hydrolysis of cell‐surface glycosphingolipids (GSLs) of intact cells by endoglycoceramidase (EGCase; EC.3.2.1.123) which cleaves the linkage between oligosaccharides and ceramides of various GSLs. After a 2‐h incubation of horse intact erythrocytes with 20 mU EGCase II in the presence of activator at 37°C, 68% of the N‐glycolylneuraminic‐acid‐containing ganglioside GM3(NeuGc) and 70% of 4‐O‐acetyl GM3(NeuGc) were found to be hydrolyzed without hemolysis, accompanied by a corresponding increase in ceramide but not sphingosine or N,N‐dimethylsphingosine. No hydrolysis was observed for sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, cholesterol or membrane proteins. The decrease in immunoreactivity with GMR8 antibody, specific to NeuGcα2,3Gal‐ of GM3(NeuGc), corresponded to that of cell‐surface GM3(NeuGc) by the enzyme, and almost no immunoreactivity was found when 70% of the GM3(NeuGc) was hydrolyzed. Besides the cell‐surface GM3(NeuGc) of horse erythrocytes, Gg3Cer of guinea pig, GM3(NeuAc) and LcCer of human, and bovine and rabbit erythrocyte IV3Galα‐nLc4Cer were found to be efficiently hydrolyzed by EGCase II even when present in intact cells, while human erythrocyte Gb4Cer is quite resistant to hydrolysis by the enzyme on the cell surface as well as in detergent miscelles. Glucose incorporation via the glucose transporter in erythrocytes was not affected at all by the specific and exhaustive hydrolysis of cell‐surface GSLs by EGCase II. This result strongly suggested that glucose transporter function was not directly modulated by endogenous GSLs. In summary, this paper demonstrates that, together with the assistance of activator protein, EGCase II will become a powerful tool for selectively removing sugar chains from cell‐surface GSLs without damaging other cell membrane components, and will be useful for describing the biological functions of endogenous GSLs.

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