Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells

Yoko Hashimoto, Mari Kobayashi, Etsuko Matsuzaki, Katsumasa Higashi, Fumi Takahashi-Yanaga, Aiko Takano, Masato Hirata, Fusanori Nishimura

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

In this study, we investigated the involvement of Wnt signaling in sphingosine-1-phosphate (S1P)-enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a-neutralizing antibody inhibited S1P-enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P-enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.

本文言語英語
ページ(範囲)1129-1136
ページ数8
ジャーナルCell Biology International
40
10
DOI
出版ステータス出版済み - 10 1 2016

All Science Journal Classification (ASJC) codes

  • 細胞生物学

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