We analyzed the change in the intensities of spontaneous ultraweak photon emission during the growth of the cell population of HeLa cell line using a cell culture system. Comparisons of the temporal change of intensities of spontaneous ultraweak photon emission with that of the growth curve showed that the intensity followed almost the same course with the change rate kinetics of cell population. The analysis of cell viability during the growth of the cell population indicated that there is a close relationship between the intensities of photon emission and proliferative rate of viable cells. The flow-cytometric analysis indicated that the intensities of the photon emission are related primarily to the 2n diploid cell equivalent populations. We suggest that the spontaneous ultraweak photon emission is mainly involved in the changes of the ploidy number during the proliferative process of the cancer cell line. The therapeutic effects of acupuncture may be explained scientifically by the spontaneous ultraweak photon emissions from the cells during the changes of its ploidy number in the anatomical structure of acupuncture meridians.
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