Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus

Takafumi Tomura, Hiroko Kishino, Katsumi Doi, Toshio Hara, Seiya Ogata, Satoru Kuhara

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

A plasmid, pSA1.1, of Streptomyces azureus elicited conjugative pocks and inhibited the sporulation of its host mycelia. pSA1.1 was studied with a kanamycin resistance gene derived from S. kanamyceticus as a selective marker. The deletion analysis and sequencing of the derivative plasmid found an open reading frame (ORF909b) that was involved in the sporulation-inhibitory function of pSA1.1 and in pock formation. The nucleotide sequences of ORF909b were compared with those of the genes registered in GenBank, but no similarity was found. However, the predicted amino acid sequence of ORF909b showed a significantly high similarity with that of the spoIIIE gene of Bacillus subtilis. This detected gene might be a new sporulation-regulatory gene in streptomycetes.

元の言語英語
ページ(範囲)438-443
ページ数6
ジャーナルBioscience, biotechnology, and biochemistry
57
発行部数3
DOI
出版物ステータス出版済み - 1 1 1993

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Streptomyces
Plasmids
Genes
Kanamycin Resistance
Mycelium
Nucleic Acid Databases
Regulator Genes
Bacillus subtilis
Kanamycin
Open Reading Frames
Amino Acid Sequence
Bacilli
Nucleotides
Derivatives
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus. / Tomura, Takafumi; Kishino, Hiroko; Doi, Katsumi; Hara, Toshio; Ogata, Seiya; Kuhara, Satoru.

:: Bioscience, biotechnology, and biochemistry, 巻 57, 番号 3, 01.01.1993, p. 438-443.

研究成果: ジャーナルへの寄稿記事

Tomura, Takafumi ; Kishino, Hiroko ; Doi, Katsumi ; Hara, Toshio ; Ogata, Seiya ; Kuhara, Satoru. / Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus. :: Bioscience, biotechnology, and biochemistry. 1993 ; 巻 57, 番号 3. pp. 438-443.
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abstract = "A plasmid, pSA1.1, of Streptomyces azureus elicited conjugative pocks and inhibited the sporulation of its host mycelia. pSA1.1 was studied with a kanamycin resistance gene derived from S. kanamyceticus as a selective marker. The deletion analysis and sequencing of the derivative plasmid found an open reading frame (ORF909b) that was involved in the sporulation-inhibitory function of pSA1.1 and in pock formation. The nucleotide sequences of ORF909b were compared with those of the genes registered in GenBank, but no similarity was found. However, the predicted amino acid sequence of ORF909b showed a significantly high similarity with that of the spoIIIE gene of Bacillus subtilis. This detected gene might be a new sporulation-regulatory gene in streptomycetes.",
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