SPR study for analysis of a water-soluble glycopolymer interface and molecular recognition properties

Yuhei Terada, Hirokazu Seto, Yu Hoshino, Tatsuya Murakami, Shuhei Shinohara, Kaoru Tamada, Yoshiko Miura

研究成果: Contribution to journalArticle

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Glycopolymers consisting of mannose and acrylamide with different mannose incorporation ratios (10 and 100%) were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization, and the polymer layers were prepared on a gold substrate. The detailed polymer layer structure and molecular recognition properties were analyzed by the surface plasmon resonance (SPR) technique. The glycopolymers formed pancake-like thin layers with thicknesses of ∼2 nm in air and were swollen in the aqueous solution. The molecular recognition against concanavalin A (ConA) was also analyzed by SPR. Binding constants between glycopolymers and ConA were large enough to suggest multivalent effects. Binding rate constants of ConA to glycopolymers were in the same order; however, the dissociation rate constant was lower in the glycopolymer with a mannose ratio of 100% because of the high local mannose density near the binding point of ConA.

元の言語英語
ページ(範囲)255-262
ページ数8
ジャーナルPolymer Journal
49
発行部数2
DOI
出版物ステータス出版済み - 2 1 2017

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

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