We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.
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