TY - JOUR
T1 - Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'
AU - Ishino, Yoshizumi
AU - Nomura, Yosiko
AU - Kato, Ikunoshin
PY - 1995/3/11
Y1 - 1995/3/11
N2 - We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.
AB - We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.
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U2 - 10.1093/nar/23.5.742
DO - 10.1093/nar/23.5.742
M3 - Article
C2 - 7708487
AN - SCOPUS:0028986815
VL - 23
SP - 742
EP - 744
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 5
ER -