Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

Shinichi Kuriyama, Yasushi Taguchi, Kazuto Watanabe, Kanako Nishimura, Kazutoshi Yanagibashi, Yoshiki Katayama, Takuro Niidome

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.

本文言語英語
ページ(範囲)7643-7646
ページ数4
ジャーナルBioorganic and Medicinal Chemistry
17
22
DOI
出版ステータス出版済み - 11 15 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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