Stabilization of the i-motif structure by the intra-strand cross-link formation

Kenji Kikuta, Haishun Piao, John Brazier, Yosuke Taniguchi, Kazumitsu Onizuka, Fumi Nagatsugi, Shigeki Sasaki

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

Abstract The i-motif structures are formed by oligonucleotides containing cytosine tracts under acidic conditions. The folding of the i-motif under physiological conditions is of great interest because of its biological role. In this study, we investigated the effect of the intra-strand cross-link on the stability of the i-motif structure. The 4-vinyl-substituted analog of thymidine (T-vinyl) was incorporated into the 5′-end of the human telomere complementary strand, which formed the intra-strand cross-link with the internal adenine. The intra-strand cross-linked i-motif displayed CD spectra similar to that of the natural i-motif at acidic pH, which was transformed into a random coil with the increasing pH. The pH midpoint for the transition from the i-motif to random coil increased from pH 6.1 for the natural one to pH 6.8 for the cross-linked one. The thermodynamic parameters were obtained by measuring the thermal melting behaviors by CD and UV, and it was determined that the intra-strand cross-linked i-motif is stabilized due to a favorable entropy effect. Thus, this study has clearly indicated the validity of the intra-strand cross-linking for stabilization of the i-motif structure.

本文言語英語
論文番号22754
ページ(範囲)3307-3310
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
25
16
DOI
出版ステータス出版済み - 7月 3 2015

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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