Stable and selective antiparallel type triplex DNA formation by targeting a GC base pair with the TFO containing one N2-phenyl-2-deoxyguanosine

Yosuke Taniguchi, Mei Miyazaki, Nozomu Matsueda, Lei Wang, Hidenori Okamura, Shigeki Sasaki

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The antiparallel triplex DNA is formed by the interaction between purine-rich triplex forming oligonucleotides (TFOs) and the homo-purine region within a duplex DNA. The formation of such a structure with the genome DNA promises to control the gene expression in a living cell. In this study, in an attempt to enhance the stability of the triplex DNAs, we have designed the N2-arylated deoxyguanosine derivatives. Among these analogues, we found that the TFOs containing N2-phenyl-2-deoxyguanosine (PhdG) showed a stable and selective triplex DNA formation with the GC base pair as compared to the natural dG/GC triplet. However, the multiple incorporation of PhdG into the TFOs hampered the stable triplex DNA, instead, showed a tendency to form a higher order structure. Therefore, we concluded that the stable and selective triplex DNA formation is expected by the replacement of dG by PhdG in the purine-rich TFO sequence.

本文言語英語
ページ(範囲)624-631
ページ数8
ジャーナルChemical and Pharmaceutical Bulletin
66
6
DOI
出版ステータス出版済み - 2018

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 創薬

フィンガープリント

「Stable and selective antiparallel type triplex DNA formation by targeting a GC base pair with the TFO containing one N<sup>2</sup>-phenyl-2-deoxyguanosine」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル