Staining ability and biocompatibility of brilliant blue G: Preclinical study of brilliant blue G as an adjunct for capsular staining

Toshio Hisatomi, Hiroshi Enaida, Hiroyoshi Matsumoto, Tadahisa Kagimoto, Akifumi Ueno, Yasuaki Hata, Toshiaki Kubota, Yoshinobu Goto, Tatsuro Ishibashi

研究成果: ジャーナルへの寄稿学術誌査読

55 被引用数 (Scopus)

抄録

Objective: To evaluate the effectiveness and biocompatibility of brilliant blue G (BBG) for capsular visualization for continuous curvilinear capsulorrhexis. Methods: The capsular staining ability of BBG was evaluated at graded concentrations of 10.0, 1.0, 0.5, 0.25, 0.1, and 0.01 mg/mL in enucleated pig's eyes. The biocompatibility of BBG was assessed in rat's eyes for 2 months. The eyes were analyzed using light, fluorescence, transmission electron, and scanning electron microscopy. TUNEL (terminal deoxynucleotidyl transferase-mediated biotin-deoxyuridine triphosphate nick-end labeling) was used to detect apoptotic cells, and endothelial cell counts were analyzed using scanning electron microscopy. The results were compared using indocyanine green and trypan blue. Results: The BBG improved capsular visualization, and a complete capsulorrhexis could be performed. In the rat model, no apparent toxic effect was observed using biomicroscopy during 2 months. Histologically, BBG showed satisfactory biocompatibility. Apoptotic cell death of the endothelial cells was detected in only the trypan blue group. In contrast to BBG, indocyanine green and trypan blue showed degeneration of corneal endothelial cells using transmission and scanning electron microscopy. Conclusion: The BBG contributed to better capsular visualization and caused no apparent complications to the corneal endothelium. Clinical Relevance: The BBG is effective and safe capsular staining for continuous curvilinear capsulorrhexis.

本文言語英語
ページ(範囲)514-519
ページ数6
ジャーナルArchives of Ophthalmology
124
4
DOI
出版ステータス出版済み - 2006

!!!All Science Journal Classification (ASJC) codes

  • 眼科学

フィンガープリント

「Staining ability and biocompatibility of brilliant blue G: Preclinical study of brilliant blue G as an adjunct for capsular staining」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル