Staurosporine-induced cleavage of α-smooth muscle actin during myofibroblast apoptosis

Ayako Nakazono-Kusaba, Fumi Takahashi-Yanaga, Sachio Morimoto, Masutaka Furue, Toshiyuki Sasaguri

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

To examine the possibility that staurosporine is applicable for the treatment of abnormal scar formation such as hypertrophic scar and keloid, the cellular process during staurosporine-induced apoptosis was analyzed in myofibroblasts isolated from a rat granulation tissue pouch. Staurosporine induced myofibroblast apoptosis in a time- and dose-dependent manner with typical morphologic changes. Staurosporine (1 μM) activated caspase-3 up to 3.6-fold by cleaving pro-caspase-3 (32 kDa) to active forms (17, 19, and 20 kDa). Microfilaments mainly composed of α-smooth muscle actin, a contractile protein characterizing myofibroblasts, were degraded during staurosporine-induced apoptosis. The degradation of α-smooth muscle actin bundles was detected as early as 1 h after the treatment with staurosporine. Recombinant active caspase-3 and staurosporine-stimulated caspase-3 both cleaved purified α-smooth muscle actin in vitro. These results suggested that α-smooth muscle actin is directly degraded by caspase-3 in response to apoptotic stimuli in myofibroblasts. In addition, bleomycin (100 ng per ml) and cisplatin (1 mM) also induced myofibroblast apoptosis by activating caspase-3, suggesting that these agents have a potential therapeutic value for abnormal scar formation.

元の言語英語
ページ(範囲)1008-1013
ページ数6
ジャーナルJournal of Investigative Dermatology
119
発行部数5
DOI
出版物ステータス出版済み - 1 1 2002

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Staurosporine
Myofibroblasts
Smooth Muscle
Muscle
Actins
Caspase 3
Apoptosis
Cicatrix
Hypertrophic Cicatrix
Keloid
Contractile Proteins
Granulation
Granulation Tissue
Bleomycin
Actin Cytoskeleton
Cisplatin
Rats
Tissue
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

これを引用

Staurosporine-induced cleavage of α-smooth muscle actin during myofibroblast apoptosis. / Nakazono-Kusaba, Ayako; Takahashi-Yanaga, Fumi; Morimoto, Sachio; Furue, Masutaka; Sasaguri, Toshiyuki.

:: Journal of Investigative Dermatology, 巻 119, 番号 5, 01.01.2002, p. 1008-1013.

研究成果: ジャーナルへの寄稿記事

Nakazono-Kusaba, Ayako ; Takahashi-Yanaga, Fumi ; Morimoto, Sachio ; Furue, Masutaka ; Sasaguri, Toshiyuki. / Staurosporine-induced cleavage of α-smooth muscle actin during myofibroblast apoptosis. :: Journal of Investigative Dermatology. 2002 ; 巻 119, 番号 5. pp. 1008-1013.
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