Steady-state bioluminescence of bacterial luciferase using electrochemical regeneration of flavin substrate and its application to inhibitory analysis

Shinya Yamasaki, Shinya Nakashima, Shuto Yamada, Kô Takehara

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

The model system for the biological reaction using a bacterial luciferase (BL) was developed and applied to the inhibitory analysis of the hydrophobic molecules for enzymatic reactions. The homemade flow electrochemical luminescence cell was embedded in the BL reaction system to regenerate the reduced form of the flavin mononucleotide, which is one of the substrates of the BL luminescence reaction, and to measure the luminescence intensity. The constant intensity of the continuous BL luminescence was observed using the continuous-flow BL reaction system. The proposed system was successfully applied to the inhibitory reaction of dodecaneamide on the BL luminescence reaction.

本文言語英語
ページ(範囲)67-70
ページ数4
ジャーナルBioelectrochemistry
75
1
DOI
出版ステータス出版済み - 4 1 2009

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 物理化学および理論化学
  • 電気化学

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