Steady‐state approximation of enzyme activation and inhibition

Masahiro Okamoto, Youko Takeda, Yoichi Aso, Katsuya Hayashi

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

This article deals with the effects of the initial concentration of effector (inhibitor or activator) on the steady‐state approximation of enzyme kinetics. The results could be summarized as follows: (1) In competitive inhibition, the increase in the initial concentration of inhibitor led to the reduction of steady state time. (2) In noncompetitive and uncompetitive inhibitions, the steady state time was not changed with the increase in the initial concentration of inhibitor. (3) In nonessential activation, the increase in the initial concentration of activator led to the reduction of steadystate time. (4) It was specially noted that in nonessential activation, even if the reaction is in the steady‐state, activation constant (KA) can not be determined exactly unless the initial concentration of activator is very small.

元の言語英語
ページ(範囲)1453-1463
ページ数11
ジャーナルBiotechnology and Bioengineering
25
発行部数6
DOI
出版物ステータス出版済み - 1 1 1983

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Enzyme inhibition
Enzyme Activation
Enzymes
Chemical activation
Enzyme kinetics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Steady‐state approximation of enzyme activation and inhibition. / Okamoto, Masahiro; Takeda, Youko; Aso, Yoichi; Hayashi, Katsuya.

:: Biotechnology and Bioengineering, 巻 25, 番号 6, 01.01.1983, p. 1453-1463.

研究成果: ジャーナルへの寄稿記事

Okamoto, M, Takeda, Y, Aso, Y & Hayashi, K 1983, 'Steady‐state approximation of enzyme activation and inhibition', Biotechnology and Bioengineering, 巻. 25, 番号 6, pp. 1453-1463. https://doi.org/10.1002/bit.260250604
Okamoto, Masahiro ; Takeda, Youko ; Aso, Yoichi ; Hayashi, Katsuya. / Steady‐state approximation of enzyme activation and inhibition. :: Biotechnology and Bioengineering. 1983 ; 巻 25, 番号 6. pp. 1453-1463.
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