Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus

Hirofumi Ohga, Kensuke Kaneko, Akio Shimizu, Hajime Kitano, Sethu Selvaraj, Mitsuo Nyuji, Hayato Adachi, Akihiko Yamaguchi, Michiya Matsuyama

研究成果: ジャーナルへの寄稿学術誌査読

21 被引用数 (Scopus)

抄録

Background: The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.Methods: Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.Results: Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.Conclusions: Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.

本文言語英語
論文番号71
ジャーナルReproductive Biology and Endocrinology
10
DOI
出版ステータス出版済み - 9月 5 2012

!!!All Science Journal Classification (ASJC) codes

  • 生殖医学
  • 内分泌学
  • 産婦人科学
  • 発生生物学

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