Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-β-glucosidase identified in fungi

Takashi Watanabe, Tomoharu Ito, Hatsumi M. Goda, Yohei Ishibashi, Tomofumi Miyamoto, Kazutaka Ikeda, Ryo Taguchi, Nozomu Okino, Makoto Ito

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Background: Endoglycoceramidase-related protein 1 (EGCrP1) is a glucocerebrosidase in Cryptococcus neoformans; however, the functions of its paralogue, EGCrP2, remain unknown. Results: EGCrP2, but not EGCrP1, hydrolyzed steryl-β-glucosides. Disruption of egcrp2 accumulated ergosteryl-3β-glucoside in C. neoformans. Conclusion: EGCrP2 degraded steryl-β-glucosides both in vivo and in vitro. Significance: EGCrP2 is a missing link in steryl-β-glucoside metabolism in fungi. In addition, EGCrP2 is a potential target for antifungal drugs.

元の言語英語
ページ(範囲)1005-1019
ページ数15
ジャーナルJournal of Biological Chemistry
290
発行部数2
DOI
出版物ステータス出版済み - 9 9 2015

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endoglycoceramidase
Glucosidases
Cryptococcus neoformans
Fungi
Glucosides
Proteins
Glucosylceramidase
Metabolism

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-β-glucosidase identified in fungi. / Watanabe, Takashi; Ito, Tomoharu; Goda, Hatsumi M.; Ishibashi, Yohei; Miyamoto, Tomofumi; Ikeda, Kazutaka; Taguchi, Ryo; Okino, Nozomu; Ito, Makoto.

:: Journal of Biological Chemistry, 巻 290, 番号 2, 09.09.2015, p. 1005-1019.

研究成果: ジャーナルへの寄稿記事

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abstract = "Background: Endoglycoceramidase-related protein 1 (EGCrP1) is a glucocerebrosidase in Cryptococcus neoformans; however, the functions of its paralogue, EGCrP2, remain unknown. Results: EGCrP2, but not EGCrP1, hydrolyzed steryl-β-glucosides. Disruption of egcrp2 accumulated ergosteryl-3β-glucoside in C. neoformans. Conclusion: EGCrP2 degraded steryl-β-glucosides both in vivo and in vitro. Significance: EGCrP2 is a missing link in steryl-β-glucoside metabolism in fungi. In addition, EGCrP2 is a potential target for antifungal drugs.",
author = "Takashi Watanabe and Tomoharu Ito and Goda, {Hatsumi M.} and Yohei Ishibashi and Tomofumi Miyamoto and Kazutaka Ikeda and Ryo Taguchi and Nozomu Okino and Makoto Ito",
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T1 - Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-β-glucosidase identified in fungi

AU - Watanabe, Takashi

AU - Ito, Tomoharu

AU - Goda, Hatsumi M.

AU - Ishibashi, Yohei

AU - Miyamoto, Tomofumi

AU - Ikeda, Kazutaka

AU - Taguchi, Ryo

AU - Okino, Nozomu

AU - Ito, Makoto

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AB - Background: Endoglycoceramidase-related protein 1 (EGCrP1) is a glucocerebrosidase in Cryptococcus neoformans; however, the functions of its paralogue, EGCrP2, remain unknown. Results: EGCrP2, but not EGCrP1, hydrolyzed steryl-β-glucosides. Disruption of egcrp2 accumulated ergosteryl-3β-glucoside in C. neoformans. Conclusion: EGCrP2 degraded steryl-β-glucosides both in vivo and in vitro. Significance: EGCrP2 is a missing link in steryl-β-glucoside metabolism in fungi. In addition, EGCrP2 is a potential target for antifungal drugs.

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