Stiffness-index map based on single cell-spheroid analysis using robot integrated microfluidic chiip

Keitaro Ito, Shinya Sakuma, Masaki Kimura, Takanori Takebe, Makoto Kaneko, Fumihito Arai

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

1 被引用数 (Scopus)

抄録

We present a method which can evaluate stiffness of cell-spheroids, and show the measurement results of the stiffness changes with respect to the culture time. To obtain the stiffness-index map, where the map shows the relationship between the size and stiffness of cell-spheroids, we applied on-chip measurement method using a robot integrated microfluidic chip (robochip). The robochip has a microchannel and a pair of on-chip probes which contains a force sensor. By measuring the stiffness in microfluidic channel, we can achieve a stable and high-throughput evaluation of mechanical characteristics. The stiffness of the single spheroid is evaluated from the reactive force when the target spheroid is deformed by the on-chip probes. The stiffness-index of cell-spheroid is proposed, and the evaluation results of the stiffness index shows that the index increased along the culture days. The proposed system will provide us a new index for the cell-quality evaluation.

本文言語英語
ホスト出版物のタイトルMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
出版社Institute of Electrical and Electronics Engineers Inc.
ページ157-160
ページ数4
ISBN(電子版)9781509019731
DOI
出版ステータス出版済み - 2 26 2016
外部発表はい
イベント29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, 中国
継続期間: 1 24 20161 28 2016

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2016-February
ISSN(印刷版)1084-6999

その他

その他29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
国/地域中国
CityShanghai
Period1/24/161/28/16

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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