Stimulation of all T cells bearing Vβ1, Vβ3, Vβ11 and Vβ12 by staphylococcal enterotoxin A

Hiroaki Takimoto, Yasunobu Yoshikai, Kenji Kishihara, Goro Matsuzaki, Hiroshi Kuga, Tsuyoshi Otani, Kikuo Nomoto

    研究成果: ジャーナルへの寄稿記事

    68 引用 (Scopus)

    抜粋

    To determine the molecular mechanisms of T cell stimulation by staphylococcal enterotoxin A (SEA), we examined the expression of T cell receptor (TcR) Vβ on the T cells from four strains of mice stimulated in vitro with SEA, using flow cytometric analysis for the number of T cells bearing Vβ3, Vβ6, Vβ8, Vβ11 and RNA blotting analysis for the amount of transcripts of Vβ1, Vβ5 and Vβ12. The number of T cell blasts bearing Vβ1, Vβ3, Vβ1 or Vβ12 were increased in the T cell blasts proliferating in vitro in response to SEA in C57BL/6 mice. In AKR/J mice, which contain few Vβ11‐ or Vβ12‐bearing T cells due to a tolerance to the self‐MHC class II IE‐antigens, T cells bearing Vβ1 or Vβ3 responded to SEA. SEA enriched only Vβ1‐bearing T cells in BALB/c mice carrying Mls‐2a which lack Mls‐1a‐reactive Vβ3‐bearing T cells as well as Vβ11‐ and Vβ12‐bearing T cells. In spite of the presence of Vβ1‐bearing T cells, C3H/He T cells exhibited a very low responsiveness to SEA. T cell repertoires skewed by clonal deletion of self‐reactive T cells may in part account for the different sensitivity to SEA among the different strains. A tolerance to SEA can be established in C57BL/6 mice which have been primed i.v. with SEA and treated i.p. with 200 mg/kg of cyclophosphamide 2 days later. All mature T cells bearing Vβ3 or Vβ11 were virtually abolished in the periphery of tolerant mice. These results suggest that most T cells reactive to SEA bear Vβ1, Vβ3, Vβ11 or Vβ12 and that clonal deletion of mature T cells reactive to SEA may account for the cellular mechanisms for cyclophosphamide‐induced tolerance to SEA.

    元の言語英語
    ページ(範囲)617-621
    ページ数5
    ジャーナルEuropean Journal of Immunology
    20
    発行部数3
    DOI
    出版物ステータス出版済み - 3 1990

      フィンガープリント

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    これを引用

    Takimoto, H., Yoshikai, Y., Kishihara, K., Matsuzaki, G., Kuga, H., Otani, T., & Nomoto, K. (1990). Stimulation of all T cells bearing Vβ1, Vβ3, Vβ11 and Vβ12 by staphylococcal enterotoxin A. European Journal of Immunology, 20(3), 617-621. https://doi.org/10.1002/eji.1830200323