TY - JOUR
T1 - Strategies and development of molecular probes for nitrogen monoxide monitoring
AU - Katayama, Yoshiki
AU - Soh, Nobuaki
AU - Maeda, Mizuo
PY - 2002/8/1
Y1 - 2002/8/1
N2 - Current methods for monitoring nitrogen monoxide (NO) and new strategies for designing NO probes are reviewed. The production of endogenous NO must be monitored if we are to understand the physiological and pathological roles of NO. Many methods have been reported for this purpose, including colorimetry, fluorometry, electrochemical methods, and electron paramagnetic resonance (EPR). Among these methods, one of the most promising for practical use is EPR using (dithiocarbamato)iron(II) complex as a spin trap. In the structure-sensitivity relationship, it was found that an electron-donating group on the dithiocarbamato ligand is favorable for enhancing the sensitivity of NO detection due to the stabilization of the Fe(II) complex form. A new concept, "radical-exchange", is also introduced to solve the issue of low sensitivity in EPR methods. This concept has been used to develop two types of NO probe: spin trap and fluorescent probes. These reagents detect NO to a detection limit of 10 nM-sub-μM. This strategy, using radical exchange in conjunction with EPR, is potentially useful for the design of sensitive probes that can detect NO directly.
AB - Current methods for monitoring nitrogen monoxide (NO) and new strategies for designing NO probes are reviewed. The production of endogenous NO must be monitored if we are to understand the physiological and pathological roles of NO. Many methods have been reported for this purpose, including colorimetry, fluorometry, electrochemical methods, and electron paramagnetic resonance (EPR). Among these methods, one of the most promising for practical use is EPR using (dithiocarbamato)iron(II) complex as a spin trap. In the structure-sensitivity relationship, it was found that an electron-donating group on the dithiocarbamato ligand is favorable for enhancing the sensitivity of NO detection due to the stabilization of the Fe(II) complex form. A new concept, "radical-exchange", is also introduced to solve the issue of low sensitivity in EPR methods. This concept has been used to develop two types of NO probe: spin trap and fluorescent probes. These reagents detect NO to a detection limit of 10 nM-sub-μM. This strategy, using radical exchange in conjunction with EPR, is potentially useful for the design of sensitive probes that can detect NO directly.
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U2 - 10.1246/bcsj.75.1681
DO - 10.1246/bcsj.75.1681
M3 - Article
AN - SCOPUS:0036349966
VL - 75
SP - 1681
EP - 1691
JO - Bulletin of the Chemical Society of Japan
JF - Bulletin of the Chemical Society of Japan
SN - 0009-2673
IS - 8
ER -