Streptomyces atp nucleotide 3′-pyrophosphokinase–gene cloning and sequence analysis

Toshio Higuchi, Takeshi Mikuniya, Kazutoyo Osoegawa, Satoshi Ezaki, Hiroshi Sumichika, Yoshiharu Mizui, Toshiharu Shoji, Kenji Kishihara, Shigeru Muta, Satoru Kuhara, Jim Ichiro Mukai

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

Streptomyces ATP nucleotide 3′-pyrophosphokinase is an extracellular enzyme that transfers 5′-β,γ-pyrophosphoryl groups of ATP to a variety of nucleotides at the 3′-OH site. The enzyme gene was cloned from partially Sau3AI-digested chromosomal DNA of S. morookaensis in S. lividans TK24/pIJ699 and then in E. coli JM83/pUC12. Some transformants produced the active enzyme. The gene was sequenced by the dideoxynucleotide termination procedure. Its GC content was 72%. Its putative promoter regions, showing little homology to that of the Streptomyces consensus type, were pointed out. No sequence homology was found between the pyrophosphokinase and any other known genes including those of the most mechanistically similar bacterial stringent factor and related proteins. Northern hybridization analysis showed that the gene is constitutionally polycistronic and expressed under transcriptional control. Nuclease S1 mapping indicated that the gene transcription starts from its translation initiation site.

本文言語英語
ページ(範囲)2182-2187
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
58
12
DOI
出版ステータス出版済み - 1月 1994

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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