Streptomyces nucleotide 3′-pyrophosphokinase. Synthesis of deoxynucleotide-3′-pyrophosphates and 3′-pyrophosphoryl→3′-OH transfer activity

Jun Ichiro Mukai, Abdur Razzaque, Toshio Kukita, Sawao Murao, Toyokazu Nishino

研究成果: ジャーナルへの寄稿記事

抄録

NucleoCide-3′-pyrophosphoklnase(ATP:nucleoside-S’-phosphate pyrophosphotransferase, E.C.2.7.6.4) of Streptomyces adephospholyticus has been shown to transfer 5′-ß,γ-pyrophosphoryl group of either ATP, dATP or adenosine-5′- tri-3′-diphosphate(pppApp) to 3′-slte of a variety of nucleotldlc substances including both purine and pyrimidine ribomononucleoside-5′-phosphates, short oligoribonucleotides, tRNA and 5′-dl- or triphosphonucleosidic sugars and their derivatives such as GDP-glucose, UDP-glucose, CDP-choline, NAD, FAD and AG-5′-ppp-5′ - Aeb, a cap nucleotide at the 5′-terminus of eucaryote raRNAs. The present paper reports two newly found facts of this enzyme: 1) deoxyATP(pppdA) is active not only as a donor but also as an acceptor of the pyrophosphoryl group giving rise to its 3′-pyrophosphoryl derivative, pppdApp. pppdGpp could also be prepared using dGTP as the acceptor. 2) the enzyme catalyses both 5′- P, y-pyrophosphoryl-β3′-OH kinase reaction(irreversible) and 3′-a,ß-pyrophos- phoryl↔3′-OH transfer reaction(reversible).

元の言語英語
ページ(範囲)S451-s456
ジャーナルNucleic acids research
1
DOI
出版物ステータス出版済み - 1 1 1978

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nucleotide pyrophosphokinase
Diphosphates
Streptomyces
Diphosphotransferases
Adenosine Triphosphate
Phosphates
Oligoribonucleotides
Cytidine Diphosphate Choline
Uridine Diphosphate Glucose
Flavin-Adenine Dinucleotide
Enzymes
Transfer RNA
Nucleosides
NAD
Adenosine
Phosphotransferases
Nucleotides

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Streptomyces nucleotide 3′-pyrophosphokinase. Synthesis of deoxynucleotide-3′-pyrophosphates and 3′-pyrophosphoryl→3′-OH transfer activity. / Mukai, Jun Ichiro; Razzaque, Abdur; Kukita, Toshio; Murao, Sawao; Nishino, Toyokazu.

:: Nucleic acids research, 巻 1, 01.01.1978, p. S451-s456.

研究成果: ジャーナルへの寄稿記事

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title = "Streptomyces nucleotide 3′-pyrophosphokinase. Synthesis of deoxynucleotide-3′-pyrophosphates and 3′-pyrophosphoryl→3′-OH transfer activity",
abstract = "NucleoCide-3′-pyrophosphoklnase(ATP:nucleoside-S’-phosphate pyrophosphotransferase, E.C.2.7.6.4) of Streptomyces adephospholyticus has been shown to transfer 5′-{\ss},γ-pyrophosphoryl group of either ATP, dATP or adenosine-5′- tri-3′-diphosphate(pppApp) to 3′-slte of a variety of nucleotldlc substances including both purine and pyrimidine ribomononucleoside-5′-phosphates, short oligoribonucleotides, tRNA and 5′-dl- or triphosphonucleosidic sugars and their derivatives such as GDP-glucose, UDP-glucose, CDP-choline, NAD, FAD and AG-5′-ppp-5′ - Aeb, a cap nucleotide at the 5′-terminus of eucaryote raRNAs. The present paper reports two newly found facts of this enzyme: 1) deoxyATP(pppdA) is active not only as a donor but also as an acceptor of the pyrophosphoryl group giving rise to its 3′-pyrophosphoryl derivative, pppdApp. pppdGpp could also be prepared using dGTP as the acceptor. 2) the enzyme catalyses both 5′- P, y-pyrophosphoryl-β3′-OH kinase reaction(irreversible) and 3′-a,{\ss}-pyrophos- phoryl↔3′-OH transfer reaction(reversible).",
author = "Mukai, {Jun Ichiro} and Abdur Razzaque and Toshio Kukita and Sawao Murao and Toyokazu Nishino",
year = "1978",
month = "1",
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T1 - Streptomyces nucleotide 3′-pyrophosphokinase. Synthesis of deoxynucleotide-3′-pyrophosphates and 3′-pyrophosphoryl→3′-OH transfer activity

AU - Mukai, Jun Ichiro

AU - Razzaque, Abdur

AU - Kukita, Toshio

AU - Murao, Sawao

AU - Nishino, Toyokazu

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N2 - NucleoCide-3′-pyrophosphoklnase(ATP:nucleoside-S’-phosphate pyrophosphotransferase, E.C.2.7.6.4) of Streptomyces adephospholyticus has been shown to transfer 5′-ß,γ-pyrophosphoryl group of either ATP, dATP or adenosine-5′- tri-3′-diphosphate(pppApp) to 3′-slte of a variety of nucleotldlc substances including both purine and pyrimidine ribomononucleoside-5′-phosphates, short oligoribonucleotides, tRNA and 5′-dl- or triphosphonucleosidic sugars and their derivatives such as GDP-glucose, UDP-glucose, CDP-choline, NAD, FAD and AG-5′-ppp-5′ - Aeb, a cap nucleotide at the 5′-terminus of eucaryote raRNAs. The present paper reports two newly found facts of this enzyme: 1) deoxyATP(pppdA) is active not only as a donor but also as an acceptor of the pyrophosphoryl group giving rise to its 3′-pyrophosphoryl derivative, pppdApp. pppdGpp could also be prepared using dGTP as the acceptor. 2) the enzyme catalyses both 5′- P, y-pyrophosphoryl-β3′-OH kinase reaction(irreversible) and 3′-a,ß-pyrophos- phoryl↔3′-OH transfer reaction(reversible).

AB - NucleoCide-3′-pyrophosphoklnase(ATP:nucleoside-S’-phosphate pyrophosphotransferase, E.C.2.7.6.4) of Streptomyces adephospholyticus has been shown to transfer 5′-ß,γ-pyrophosphoryl group of either ATP, dATP or adenosine-5′- tri-3′-diphosphate(pppApp) to 3′-slte of a variety of nucleotldlc substances including both purine and pyrimidine ribomononucleoside-5′-phosphates, short oligoribonucleotides, tRNA and 5′-dl- or triphosphonucleosidic sugars and their derivatives such as GDP-glucose, UDP-glucose, CDP-choline, NAD, FAD and AG-5′-ppp-5′ - Aeb, a cap nucleotide at the 5′-terminus of eucaryote raRNAs. The present paper reports two newly found facts of this enzyme: 1) deoxyATP(pppdA) is active not only as a donor but also as an acceptor of the pyrophosphoryl group giving rise to its 3′-pyrophosphoryl derivative, pppdApp. pppdGpp could also be prepared using dGTP as the acceptor. 2) the enzyme catalyses both 5′- P, y-pyrophosphoryl-β3′-OH kinase reaction(irreversible) and 3′-a,ß-pyrophos- phoryl↔3′-OH transfer reaction(reversible).

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