Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment

Hiroki Otsuka, Noboru Arimura, Shozo Sonoda, Makoto Nakamura, Teruto Hashiguchi, Ikuro Maruyama, Shintaro Nakao, Ali Hafezi-Moghadam, Taiji Sakamoto

研究成果: Contribution to journalArticle査読

25 被引用数 (Scopus)

抄録

Stromal cell-derived factor-1 (SDF-1) causes chemotaxis of CXCR4-expressing bone marrow-derived cells. SDF-1 is involved in the pathogenesis of various vascular diseases, including those of the eye. However, the role of SDF-1 in neuronal diseases is not completely understood. Here, we show higher SDF-1 levels in the vitreous humor of patients with retinal detachment (RD) compared with normal patients. SDF-1 correlated positively with the duration as well as the extent of RD. Furthermore, SDF-1 correlated significantly with levels of interleukin-6 and interleukin-8, but not with vascular endothelial growth factor. Western blot analysis results showed significant SDF-1 up-regulation in detached rat retinas compared with normal animals. Immunohistochemistry data showed that SDF-1 was co-localized with the glial cells of the detached retina. SDF-1 blockade with a neutralizing antibody increased photoreceptor cell loss and macrophage accumulation in the subretinal space. The retinal precursor cell line R28 expressed CXCR4. SDF-1 rescued serum starvation-induced apoptosis in R28 cells and enhanced their ability to participate in wound closure in a scratch assay. Our results indicate a surprising, protective role for SDF-1 in RD. This effect may be mediated directly or indirectly through other cell types.

本文言語英語
ページ(範囲)2268-2277
ページ数10
ジャーナルAmerican Journal of Pathology
177
5
DOI
出版ステータス出版済み - 11 2010

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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