Structural analysis of ribosomal dna homologues in nucleolus-less mutant of Xenopus laevis

Tashiro Kosuke, Shiokawa Koichiro, Yamana Kiyotaka, Sakaki Yoshiyuki

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Sequences homologous to the ribosomal DNA (rDNA) m a Xenopus anucleolate (nucleolus-less) mutant were analyzed by Southern blot analysis. The mutant was found to possess a variety of sequences homologous to non-transcribed spacer (NTS) and/or coding region of rDNA. 65 rDNA-homologous clones were isolated from a genomic DNA library of the mutant. All the clones showed only partial homology to the normal rDNA unit and their restriction maps differed from that of the normal rDNA unit. Based on the hybridization patterns, the rDNA-homologous clones were divided into four groups (I-IV). Structure of group IV, which most strongly hybridized to normal rDNA probe, was analyzed by nucleotide sequencing. The group IV sequence was found to contain a part of the rDNA, including Bam island, enhancer element, promoter region, external transcribed spacer, and a portion of 18 S rRNA gene. The blotting analysis suggested that the group IV sequence is specific for a particular strain of Xenopus.

元の言語英語
ページ(範囲)299-306
ページ数8
ジャーナルGene
44
発行部数2-3
DOI
出版物ステータス出版済み - 1986

Fingerprint

Xenopus laevis
Ribosomal DNA
Clone Cells
Sequence Homology
Xenopus
Genomic Library
DNA Probes
Southern Blotting
Gene Library
rRNA Genes
Islands
Genetic Promoter Regions
Nucleotides

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Structural analysis of ribosomal dna homologues in nucleolus-less mutant of Xenopus laevis. / Kosuke, Tashiro; Koichiro, Shiokawa; Kiyotaka, Yamana; Yoshiyuki, Sakaki.

:: Gene, 巻 44, 番号 2-3, 1986, p. 299-306.

研究成果: ジャーナルへの寄稿記事

Kosuke, Tashiro ; Koichiro, Shiokawa ; Kiyotaka, Yamana ; Yoshiyuki, Sakaki. / Structural analysis of ribosomal dna homologues in nucleolus-less mutant of Xenopus laevis. :: Gene. 1986 ; 巻 44, 番号 2-3. pp. 299-306.
@article{5522c39ffb4144f4b2283d418526e653,
title = "Structural analysis of ribosomal dna homologues in nucleolus-less mutant of Xenopus laevis",
abstract = "Sequences homologous to the ribosomal DNA (rDNA) m a Xenopus anucleolate (nucleolus-less) mutant were analyzed by Southern blot analysis. The mutant was found to possess a variety of sequences homologous to non-transcribed spacer (NTS) and/or coding region of rDNA. 65 rDNA-homologous clones were isolated from a genomic DNA library of the mutant. All the clones showed only partial homology to the normal rDNA unit and their restriction maps differed from that of the normal rDNA unit. Based on the hybridization patterns, the rDNA-homologous clones were divided into four groups (I-IV). Structure of group IV, which most strongly hybridized to normal rDNA probe, was analyzed by nucleotide sequencing. The group IV sequence was found to contain a part of the rDNA, including Bam island, enhancer element, promoter region, external transcribed spacer, and a portion of 18 S rRNA gene. The blotting analysis suggested that the group IV sequence is specific for a particular strain of Xenopus.",
author = "Tashiro Kosuke and Shiokawa Koichiro and Yamana Kiyotaka and Sakaki Yoshiyuki",
year = "1986",
doi = "10.1016/0378-1119(86)90194-0",
language = "English",
volume = "44",
pages = "299--306",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Structural analysis of ribosomal dna homologues in nucleolus-less mutant of Xenopus laevis

AU - Kosuke, Tashiro

AU - Koichiro, Shiokawa

AU - Kiyotaka, Yamana

AU - Yoshiyuki, Sakaki

PY - 1986

Y1 - 1986

N2 - Sequences homologous to the ribosomal DNA (rDNA) m a Xenopus anucleolate (nucleolus-less) mutant were analyzed by Southern blot analysis. The mutant was found to possess a variety of sequences homologous to non-transcribed spacer (NTS) and/or coding region of rDNA. 65 rDNA-homologous clones were isolated from a genomic DNA library of the mutant. All the clones showed only partial homology to the normal rDNA unit and their restriction maps differed from that of the normal rDNA unit. Based on the hybridization patterns, the rDNA-homologous clones were divided into four groups (I-IV). Structure of group IV, which most strongly hybridized to normal rDNA probe, was analyzed by nucleotide sequencing. The group IV sequence was found to contain a part of the rDNA, including Bam island, enhancer element, promoter region, external transcribed spacer, and a portion of 18 S rRNA gene. The blotting analysis suggested that the group IV sequence is specific for a particular strain of Xenopus.

AB - Sequences homologous to the ribosomal DNA (rDNA) m a Xenopus anucleolate (nucleolus-less) mutant were analyzed by Southern blot analysis. The mutant was found to possess a variety of sequences homologous to non-transcribed spacer (NTS) and/or coding region of rDNA. 65 rDNA-homologous clones were isolated from a genomic DNA library of the mutant. All the clones showed only partial homology to the normal rDNA unit and their restriction maps differed from that of the normal rDNA unit. Based on the hybridization patterns, the rDNA-homologous clones were divided into four groups (I-IV). Structure of group IV, which most strongly hybridized to normal rDNA probe, was analyzed by nucleotide sequencing. The group IV sequence was found to contain a part of the rDNA, including Bam island, enhancer element, promoter region, external transcribed spacer, and a portion of 18 S rRNA gene. The blotting analysis suggested that the group IV sequence is specific for a particular strain of Xenopus.

UR - http://www.scopus.com/inward/record.url?scp=0022514828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022514828&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(86)90194-0

DO - 10.1016/0378-1119(86)90194-0

M3 - Article

C2 - 3023192

AN - SCOPUS:0022514828

VL - 44

SP - 299

EP - 306

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2-3

ER -