Structural and biochemical characterization of the C 3-C 4 intermediate Brassica gravinae and relatives, with particular reference to cellular distribution of Rubisco

Osamu Ueno

研究成果: Contribution to journalArticle査読

18 被引用数 (Scopus)

抄録

On the basis of its CO 2 compensation concentration, Brassica gravinae Ten. has been reported to be a C 3-C 4 intermediate. This study investigated the structural and biochemical features of photosynthetic metabolism in B. gravinae. The cellular distribution of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) was also examined in B. gravinae, B. napus L. (C 3), Raphanus sativus L. (C 3), and Diplotaxis tenuifolia (L.) DC. (C 3-C 4) by immunogold electron microscopy to elucidate Rubisco expression during the evolution from C 3 to C 3-C 4 intermediate plants. The bundle sheath (BS) cells of B. gravinae contained centrifugally located chloroplasts as well as centripetally located chloroplasts and mitochondria. Glycine decarboxylase P-protein was localized in the BS mitochondria. Brassica gravinae had low C 4 enzyme activities and high activities of Rubisco and photorespiratory enzymes, suggesting that it reduces photorespiratory CO 2 loss by the glycine shuttle. In B. gravinae, the labelling density of Rubisco was higher in the mesophyll chloroplasts than in the BS chloroplasts. A similar cellular pattern was found in other Brassicaceae species. These data demonstrate that, during the evolution from C 3 to C 3-C 4 intermediate plants, the intercellular pattern of Rubisco expression did not change greatly, although the amount of chloroplasts in the BS cells increased. It also appears that intracellular variation in Rubisco distribution may occur within the BS cells of B. gravinae.

本文言語英語
ページ(範囲)5347-5355
ページ数9
ジャーナルJournal of Experimental Botany
62
15
DOI
出版ステータス出版済み - 11 2011

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

フィンガープリント 「Structural and biochemical characterization of the C <sub>3</sub>-C <sub>4</sub> intermediate Brassica gravinae and relatives, with particular reference to cellular distribution of Rubisco」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル